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IgA Monoclonal antibody

Human IgA Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, IHC, IF-P, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

3A5A5

Cat no : 60099-1-Ig

Print datasheet

Synonyms

Human IgA, IgA1, IGHA1

验证数据展示

  • WB analysis of human saliva using 60099-1-Ig

    WB analysis of human saliva using 60099-1-Ig

    human saliva were subjected to SDS PAGE followed by western blot with 60099-1-Ig (Human IgA Antibody) at dilution of 1:16000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 60099-1-Ig

    IHC staining of human tonsillitis using 60099-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60099-1-Ig (Human IgA antibody) at dilution of 1:320000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Human IgA antibody included
  • IF Staining of human tonsillitis using 60099-1-Ig

    IF Staining of human tonsillitis using 60099-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60099-1-Ig (Human IgA antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inhuman saliva tissue, human saliva, human plasma
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Planning an IHC experiment? We recommend our IHCeasy Human IgA Ready-To-Use IHC Kit. Human IgA primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:160000-1:640000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

60099-1-Ig targets Human IgA in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human
Cited Reactivityrat
Immunogen Human IgA fusion protein Ag7417 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name immunoglobulin heavy constant alpha 1
Synonyms Human IgA, IgA1, IGHA1
Calculated Molecular Weight 496 aa, 53 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC016369
Gene Symbol Human IgA Heavy Chain
Gene ID (NCBI) 3493
RRIDAB_1557448
Conjugate Unconjugated
Form Liquid
Purification MethodCaprylic acid/ammonium sulfate precipitation
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. This antibody recognizes the Chain C region of human Ig alpha-1 (IGHA1).

实验方案

Product Specific Protocols
WB protocol for Human IgA antibody 60099-1-IgDownload protocol
IHC protocol for Human IgA antibody 60099-1-IgDownload protocol
IF protocol for Human IgA antibody 60099-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

J Nanobiotechnology

A milk-based self-assemble rotavirus VP6-ferritin nanoparticle vaccine elicited protection against the viral infection.

Authors - Zhipeng Li
View Article
ratIF

Eur J Pharmacol

Inhibition of excessive autophagy alleviates renal injury and inflammation in a rat model of immunoglobulin A nephropathy

Authors - Meiying Chang
View Article