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验证数据展示

  • IHC staining of human tonsillitis using Biotin-60099

    IHC staining of human tonsillitis using Biotin-60099

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using Biotin-60099 (Human IgA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Human IgA antibody included

Biotin-conjugated IgA Monoclonal antibody

Human IgA Monoclonal Antibody for IHC
Cat No. Biotin-60099
CloneNo. 3A5A5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC

Synonyms

FLJ14473, FLJ35065, FLJ35500, FLJ36402, FLJ39698, FLJ40001, FLJ41548, FLJ41552, FLJ41789, FLJ43248, FLJ43594, FLJ44293, FLJ46028, FLJ46621, FLJ46724, FLJ46811, FLJ46824, FLJ90170, IgA1, IGHA1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Biotin
Size: 

-/ -

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经过测试的应用

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy Human IgA Ready-To-Use IHC Kit. Human IgA primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-60099 targets Human IgA in IHC applications and shows reactivity with human samples.

Tested Applications IHC Application Description
Tested Reactivity human
Immunogen Human IgA fusion protein Ag7417 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name immunoglobulin heavy constant alpha 1
Synonyms FLJ14473, FLJ35065, FLJ35500, FLJ36402, FLJ39698, FLJ40001, FLJ41548, FLJ41552, FLJ41789, FLJ43248, FLJ43594, FLJ44293, FLJ46028, FLJ46621, FLJ46724, FLJ46811, FLJ46824, FLJ90170, IgA1, IGHA1
Calculated Molecular Weight 496 aa, 53 kDa
Observed Molecular Weight 55-60 kDa
GenBank Accession NumberBC016369
Gene Symbol Human IgA Heavy Chain
Gene ID (NCBI) 3493
RRIDAB_2918945
Conjugate Biotin
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ig alpha is the major immunoglobulin class in body secretions. It may serve both to defend against local infection and to prevent access of foreign antigens to the general immunologic system. This antibody recognizes the Chain C region of human Ig alpha-1 (IGHA1).

实验方案

Product Specific Protocols
IHC protocol for Biotin Human IgA antibody Biotin-60099Download protocol
Standard Protocols
Click here to view our Standard Protocols