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N-cadherin Monoclonal antibody
N-cadherin Monoclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, pig, mouse, rat, rabbit and More (1)
Applications
WB, IHC, IF-P, ELISA and More (1)
Conjugate
Unconjugated
175
CloneNo.
1D8B3
验证数据展示
经过测试的应用
| Positive WB detected in | rabbit brain tissue, pig brain tissue, human heart tissue, MKN-45 cells, HEK-293 cells, PC-3 cells, mouse brain tissue, rat brain tissue, C2C12 cells, C6 cells, Saos-2 cells, SGC-7901 cells, ROS1728 cells, NIH/3T3 cells |
| Positive IHC detected in | mouse heart tissue, mouse brain tissue, human malignant melanoma tissue, human ovary tumor tissue, rat heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
Planning an IHC experiment? We recommend our IHCeasy N-cadherin Ready-To-Use IHC Kit. N-cadherin primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:7500-1:30000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66219-1-Ig targets N-cadherin in WB, IHC, IF-P, ELISA applications and shows reactivity with human, pig, mouse, rat, rabbit samples.
| Tested Applications | WB, IHC, IF-P, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, pig, mouse, rat, rabbit |
| Cited Reactivity | human, mouse, rat, pig, monkey |
| Immunogen | N-cadherin fusion protein Ag4996 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | cadherin 2, type 1, N-cadherin (neuronal) |
| Synonyms | Cadherin 2, CD325, CDH2, CDHN, CDw325, N cadherin, NCAD, N-cadherin, Neural cadherin |
| Calculated Molecular Weight | 906 aa, 100 kDa |
| Observed Molecular Weight | 130 kDa |
| GenBank Accession Number | BC036470 |
| Gene Symbol | N-cadherin |
| Gene ID (NCBI) | 1000 |
| RRID | AB_2881610 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P19022 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619). The N-cadherin ectodomain can be cleaved, leading to the generation of a 90 kD N-terminal fragment (PMID: 16998833; 17028923).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for N-cadherin antibody 66219-1-Ig | Download protocol |
| IHC protocol for N-cadherin antibody 66219-1-Ig | Download protocol |
| IF protocol for N-cadherin antibody 66219-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Stem Cell Long-term engraftment and maturation of autologous iPSC-derived cardiomyocytes in two rhesus macaques | ||
Mol Cancer YTHDF2 mediates the mRNA degradation of the tumor suppressors to induce AKT phosphorylation in N6-methyladenosine-dependent way in prostate cancer. | ||
J Pineal Res Melatonin and verteporfin synergistically suppress the growth and stemness of head and neck squamous cell carcinoma through the regulation of mitochondrial dynamics | ||
Aging Cell Advanced Maternal Age-associated SIRT1 Deficiency Compromises Trophoblast Epithelial-Mesenchymal Transition through an Increase in Vimentin Acetylation. | ||
Mater Today Bio Ordered micropattern arrays fabricated by lung-derived dECM hydrogels for chemotherapeutic drug screening. |
