验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse heart tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-66219 targets N-cadherin in IF-P applications and shows reactivity with human, pig, mouse, rat, rabbit samples.
| 经测试应用 | IF-P Application Description |
| 经测试反应性 | human, pig, mouse, rat, rabbit |
| 免疫原 | N-cadherin fusion protein Ag4996 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | cadherin 2, type 1, N-cadherin (neuronal) |
| 别名 | Cadherin 2, CD325, CDH2, CDHN, CDw325, N cadherin, NCAD, Neural cadherin |
| 计算分子量 | 906 aa, 100 kDa |
| 观测分子量 | 130 kDa |
| GenBank蛋白编号 | BC036470 |
| 基因名称 | N-cadherin |
| Gene ID (NCBI) | 1000 |
| RRID | AB_3084820 |
| 偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
| 最大激发/发射波长 | 654 nm / 674 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P19022 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619). The N-cadherin ectodomain can be cleaved, leading to the generation of a 90 kD N-terminal fragment (PMID: 16998833; 17028923).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 647 N-cadherin antibody CL647-66219 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |