验证数据展示
经过测试的应用
Positive IF-P detected in | mouse heart tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-66219 targets N-cadherin in IF-P applications and shows reactivity with human, pig, mouse, rat samples.
经测试应用 | IF-P Application Description |
经测试反应性 | human, pig, mouse, rat |
免疫原 | N-cadherin fusion protein Ag4996 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | cadherin 2, type 1, N-cadherin (neuronal) |
别名 | Cadherin 2, CD325, CDH2, CDHN, CDw325, N cadherin, NCAD, N-cadherin, Neural cadherin |
计算分子量 | 906 aa, 100 kDa |
GenBank蛋白编号 | BC036470 |
基因名称 | N-cadherin |
Gene ID (NCBI) | 1000 |
RRID | AB_2883286 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | P19022 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619). The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 N-cadherin antibody CL488-66219 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |