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验证数据展示

  • IF Staining of mouse heart using CL594-66219

    IF Staining of mouse heart using CL594-66219

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite®594 N-cadherin antibody (CL594-66219, Clone: 1D8B3 ) at dilution of 1:400.

  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated N-cadherin Monoclonal antibody

N-cadherin Monoclonal Antibody for IF-P
Cat No. CL594-66219

Print datasheet

CloneNo. 1D8B3

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, mouse, rat

Applications

IF-P

Synonyms

Cadherin 2, CD325, CDH2, CDHN, CDw325, N cadherin, NCAD, N-cadherin, Neural cadherin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse heart tissue
Planning an IHC experiment? We recommend our IHCeasy N-cadherin Ready-To-Use IHC Kit. N-cadherin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66219-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66219 targets N-cadherin in IF-P applications and shows reactivity with human, pig, mouse, rat samples.

Tested Applications IF-P Application Description
Tested Reactivity human, pig, mouse, rat
Immunogen N-cadherin fusion protein Ag4996 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cadherin 2, type 1, N-cadherin (neuronal)
Synonyms Cadherin 2, CD325, CDH2, CDHN, CDw325, N cadherin, NCAD, N-cadherin, Neural cadherin
Calculated Molecular Weight 906 aa, 100 kDa
GenBank Accession NumberBC036470
Gene Symbol N-cadherin
Gene ID (NCBI) 1000
RRIDAB_2883538
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP19022
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619). The antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

实验方案

Product Specific Protocols
IF protocol for CL594 N-cadherin antibody CL594-66219Download protocol
Standard Protocols
Click here to view our Standard Protocols