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验证数据展示

  • WB analysis of HEK-293 using 66219-1-Ig (same clone as 66219-1-PBS)

    WB analysis of HEK-293 using 66219-1-Ig (same clone as 66219-1-PBS)

    WB result of N-cadherin antibody (66219-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-N-cadherin transfected HEK-293 cells. This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • WB analysis using 66219-1-Ig (same clone as 66219-1-PBS)

    WB analysis using 66219-1-Ig (same clone as 66219-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66219-1-Ig (N-cadherin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • WB analysis of PC-3 using 66219-1-Ig (same clone as 66219-1-PBS)

    WB analysis of PC-3 using 66219-1-Ig (same clone as 66219-1-PBS)

    Untreated and PNGase F-treated lysates of PC-3 cells were subjected to SDS PAGE followed by western blot with 66219-1-Ig (N-cadherin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 66219-1-Ig (same clone as 66219-1-PBS)

    IHC staining of mouse heart using 66219-1-Ig (same clone as 66219-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:15000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with N-cadherin antibody included
  • IHC staining of mouse heart using 66219-1-Ig (same clone as 66219-1-PBS)

    IHC staining of mouse heart using 66219-1-Ig (same clone as 66219-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:15000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with N-cadherin antibody included
  • IF Staining of mouse heart using 66219-1-Ig (same clone as 66219-1-PBS)

    IF Staining of mouse heart using 66219-1-Ig (same clone as 66219-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using N-cadherin antibody (66219-1-Ig, Clone: 1D8B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 66219-1-Ig (same clone as 66219-1-PBS)

    IF Staining of mouse heart using 66219-1-Ig (same clone as 66219-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using N-cadherin antibody (66219-1-Ig, Clone: 1D8B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 66219-1-Ig (same clone as 66219-1-PBS)

    IHC staining of human ovary tumor using 66219-1-Ig (same clone as 66219-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with N-cadherin antibody included
  • IHC staining of human ovary tumor using 66219-1-Ig (same clone as 66219-1-PBS)

    IHC staining of human ovary tumor using 66219-1-Ig (same clone as 66219-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with N-cadherin antibody included
  • Cytometric bead array standard curve of MP50439-2

    Cytometric bead array standard curve of MP50439-2

    Cytometric bead array standard curve of MP50439-2, N-cadherin Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66219-1-PBS. Detection antibody: 66219-2-PBS. Standard:Eg0701. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 66219-1-Ig

    Affinity and Kinetic Characterization of 66219-1-Ig

    Biolayer interferometry (BLl) kinetic assays of 66219-1-PBS against Human N-cadherin were performed. The affinity constant is 12.9 nM.

  • Featured Product
  • KD/KO验证

N-cadherin Monoclonal antibody, PBS Only (Capture)

N-cadherin Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA
Cat No. 66219-1-PBS

产品说明书

CloneNo. 1D8B3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

别名

1D8B3, Cadherin 2, Cadherin-2, CD325, CDH2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy N-cadherin Ready-To-Use IHC Kit. N-cadherin primary antibody included.

产品信息

66219-1-PBS targets N-cadherin as part of a matched antibody pair:

MP50439-2: 66219-1-PBS capture and 66219-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF-P, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原

CatNo: Ag4996

Product name: Recombinant human N-cadherin protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 33-350 aa of BC036470

Sequence: KTGFPEDVYSAVLSKDVHEGQPLLNVKFSNCNGKRKVQYESSEPADFKVDEDGMVYAVRSFPLSSEHAKFLIYAQDKETQEKWQVAVKLSLKPTLTEESVKESAEVEEIVFPRQFSKHSGHLQRQKRDWVISPINLPENSRGPFPQELVRIRSDRDKNLSLRYSVTGPGADQPPTGIFIINPISGQLSVTKPLDREQIARFHLRAHAVDINGNQVENPIDIVINVIDMNDNRPEFLHQVWNGTVPEGSKPGTYVMTVTAIDADDPNALNGMLRYRIVSQAPSTPSPNMFTINNETGDIITVAAGLDREKVQQYTLIIQ

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 cadherin 2, type 1, N-cadherin (neuronal)
别名 1D8B3, Cadherin 2, Cadherin-2, CD325, CDH2
计算分子量 906 aa, 100 kDa
观测分子量 130 kDa
GenBank蛋白编号BC036470
基因名称 N-cadherin
Gene ID (NCBI) 1000
RRIDAB_2881610
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP19022
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619). The N-cadherin ectodomain can be cleaved, leading to the generation of a 90 kD N-terminal fragment (PMID: 16998833; 17028923).

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