• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

N-cadherin Monoclonal antibody, PBS Only (Capture)

N-cadherin Monoclonal Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D8B3

Cat no : 66219-1-PBS

Print datasheet

Synonyms

CDHN, CDH2, CD325, Cadherin-2, Cadherin 2

验证数据展示

  • WB analysis of HEK-293 using 66219-1-Ig (same clone as 66219-1-PBS)

    WB analysis of HEK-293 using 66219-1-Ig (same clone as 66219-1-PBS)

    WB result of N-cadherin antibody (66219-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-N-cadherin transfected HEK-293 cells. This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • WB analysis using 66219-1-Ig (same clone as 66219-1-PBS)

    WB analysis using 66219-1-Ig (same clone as 66219-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66219-1-Ig (N-cadherin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • WB analysis of PC-3 using 66219-1-Ig (same clone as 66219-1-PBS)

    WB analysis of PC-3 using 66219-1-Ig (same clone as 66219-1-PBS)

    Untreated and PNGase F-treated lysates of PC-3 cells were subjected to SDS PAGE followed by western blot with 66219-1-Ig (N-cadherin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 66219-1-Ig (same clone as 66219-1-PBS)

    IHC staining of mouse heart using 66219-1-Ig (same clone as 66219-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:15000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with N-cadherin antibody included
  • IHC staining of mouse heart using 66219-1-Ig (same clone as 66219-1-PBS)

    IHC staining of mouse heart using 66219-1-Ig (same clone as 66219-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:15000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with N-cadherin antibody included
  • IF Staining of mouse heart using 66219-1-Ig (same clone as 66219-1-PBS)

    IF Staining of mouse heart using 66219-1-Ig (same clone as 66219-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 66219-1-Ig (N-cadherin antibody) at dilution of 1:100 and CoraLite594-Conjugated Goat Anti-Mouse IgG(H+L). The section was co-stained with 11313-2-AP (alpha Actinin) in green. This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 66219-1-Ig (same clone as 66219-1-PBS)

    IHC staining of human ovary tumor using 66219-1-Ig (same clone as 66219-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with N-cadherin antibody included
  • IHC staining of human ovary tumor using 66219-1-Ig (same clone as 66219-1-PBS)

    IHC staining of human ovary tumor using 66219-1-Ig (same clone as 66219-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66219-1-Ig (N-cadherin antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66219-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with N-cadherin antibody included
  • Cytometric bead array standard curve of MP50439-2

    Cytometric bead array standard curve of MP50439-2

    Cytometric bead array standard curve of MP50439-2, N-cadherin Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66219-1-PBS. Detection antibody: 66219-2-PBS. Standard:Eg0701. Range: 0.781-100 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy N-cadherin Ready-To-Use IHC Kit. N-cadherin primary antibody included.

产品信息

66219-1-PBS targets N-cadherin as part of a matched antibody pair:

MP50439-2: 66219-1-PBS capture and 66219-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF-P, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen N-cadherin fusion protein Ag4996 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cadherin 2, type 1, N-cadherin (neuronal)
Synonyms CDHN, CDH2, CD325, Cadherin-2, Cadherin 2
Calculated Molecular Weight 906 aa, 100 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC036470
Gene Symbol N-cadherin
Gene ID (NCBI) 1000
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP19022
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. N-cadherin (neural cadherin), also known as CDH2 (cadherin 2), is a 130-kDa transmembrane protein and a classical member of the cadherin superfamily which also include E-, P-, R-, and B-cadherins. Expression of N-cadherin has been reported on various cell types including neurons, endothelial cells and cardiac myocytes (PMID: 11282032; 9508779; 8125202). N-cadherin has functions in early brain morphogenesis, synaptogenesis and synaptic plasticity (PMID: 23321619). The N-cadherin ectodomain can be cleaved, leading to the generation of a 90 kD N-terminal fragment (PMID: 16998833; 17028923).