NFKB2 Monoclonal antibody
NFKB2 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
6A10E9
验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, Jurkat cells, K-562 cells, Daudi cells, THP-1 cells |
| Positive IHC detected in | human liver cancer tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy NFKB2 Ready-To-Use IHC Kit. NFKB2 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66920-1-Ig targets NFKB2 in WB, IHC, ELISA applications and shows reactivity with Human samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Tested Reactivity | Human |
| Immunogen | NFKB2 fusion protein Ag28543 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100) |
| Synonyms | DNA binding factor KBF2, H2TF1, LYT 10, LYT10, NFKB2, NFKB2,p100, Oncogene Lyt 10, p100 |
| Calculated Molecular Weight | 97 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | BC002844 |
| Gene Symbol | NFKB2 |
| Gene ID (NCBI) | 4791 |
| ENSEMBL Gene ID | ENSG00000077150 |
| RRID | AB_2882247 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q00653 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. P52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. P52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for NFKB2 antibody 66920-1-Ig | Download protocol |
| IHC protocol for NFKB2 antibody 66920-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
