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NFKB2 Monoclonal antibody, PBS Only

NFKB2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

6A10E9

Cat no : 66920-1-PBS

Print datasheet

Synonyms

DNA binding factor KBF2, H2TF1, LYT 10, LYT10, NFKB2, NFKB2,p100, NFKB2,p52,p100, Oncogene Lyt 10, p100, p52

验证数据展示

  • WB analysis using 66920-1-Ig (same clone as 66920-1-PBS)

    WB analysis using 66920-1-Ig (same clone as 66920-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66920-1-Ig (NFKB2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66920-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66920-1-Ig (same clone as 66920-1-PBS)

    IHC staining of human liver cancer using 66920-1-Ig (same clone as 66920-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66920-1-Ig (NFKB2 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66920-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NFKB2 antibody included
Planning an IHC experiment? We recommend our IHCeasy NFKB2 Ready-To-Use IHC Kit. NFKB2 primary antibody included.

产品信息

66920-1-PBS targets NFKB2 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human
Immunogen NFKB2 fusion protein Ag28543 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
Synonyms DNA binding factor KBF2, H2TF1, LYT 10, LYT10, NFKB2, NFKB2,p100, NFKB2,p52,p100, Oncogene Lyt 10, p100, p52
Calculated Molecular Weight 97 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC002844
Gene Symbol NFKB2
Gene ID (NCBI) 4791
ENSEMBL Gene IDENSG00000077150
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ00653
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. P52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. P52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65.