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CD61 / Integrin beta 3 Monoclonal antibody
CD61 / Integrin beta 3 Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human and More (2)
Applications
WB, IHC, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
3D8E5
验证数据展示
经过测试的应用
| Positive WB detected in | HUVEC cells, human placenta tissue |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy ITGB3/CD61 Ready-To-Use IHC Kit. ITGB3/CD61 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66952-1-Ig targets CD61 / Integrin beta 3 in WB, IP, IHC, ELISA applications and shows reactivity with Human samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IP, IHC |
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse |
| Immunogen | CD61 / Integrin beta 3 fusion protein Ag27834 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61) |
| Synonyms | CD61, GP3A, GPIIIa, Integrin beta 3, ITGB3 |
| Calculated Molecular Weight | 788 aa, 87 kDa |
| Observed Molecular Weight | 100-110 kDa |
| GenBank Accession Number | BC127666 |
| Gene Symbol | Integrin beta 3 |
| Gene ID (NCBI) | 3690 |
| RRID | AB_2882275 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P05106 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Integrin beta-3, also named as CD61 and GPIIIa, is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin beta-3 recognize the sequence R-G-D in a wide array of ligands and the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen which leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. Human integrin beta-3 has a calculated molecular weight of 87 kDa. As a result of glycosylation, the apparent molecular mass of integrin beta-3 is approximately 90-110 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CD61 / Integrin beta 3 antibody 66952-1-Ig | Download protocol |
| IHC protocol for CD61 / Integrin beta 3 antibody 66952-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Rep Periostin secreted by activated fibroblasts in idiopathic pulmonary fibrosis promotes tumorigenesis of non-small cell lung cancer.
| ||
Reprod Biol Endocrinol Expression of Talin-1 in endometriosis and its possible role in pathogenesis. | ||
BMC Biotechnol αvβ3-targeted sEVs for efficient intracellular delivery of proteins using MFG-E8. | ||
Acta Pharmacol Sin Adiponectin receptor agonist AdipoRon modulates human and mouse platelet function. | ||
Drug Deliv Suppression for lung metastasis by depletion of collagen I and lysyl oxidase via losartan assisted with paclitaxel-loaded pH-sensitive liposomes in breast cancer | ||
Biomedicines ITCH-Mediated Ubiquitylation of ITGB3 Promotes Cell Proliferation and Invasion of Ectopic Endometrial Stromal Cells in Ovarian Endometriosis |
