验证数据展示
经过测试的应用
| Positive WB detected in | Ag30688 |
| Positive Neutralization detected in | Recombinant protein |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| NEUTRALIZATION | NEUTRALIZATION : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 9 publications below |
| IF | See 1 publications below |
产品信息
67758-1-Ig targets SARS-CoV-2 S protein (RBD, 319-541 aa) in WB, IF, Neutralization, ELISA applications and shows reactivity with virus samples.
| 经测试应用 | WB, Neutralization, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | virus |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag30688 Product name: Recombinant virus spike protein protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 319-541 aa of NC_045512 Sequence: RVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNF 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | SARS-CoV-2 Spike Protein |
| 别名 | COVID-19 RBD, RBD, Spike, spike protein, S protein |
| 计算分子量 | 141 kDa |
| GenBank蛋白编号 | NC_045512 |
| 基因名称 | SARS-COV-2 |
| Gene ID (NCBI) | 43740568 |
| RRID | AB_2918526 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Coronaviruses (CoVs) infect human and animals and cause varieties of diseases, including respiratory, enteric, renal, and neurological diseases. CoV uses its spike protein to recognize ACE2 as its receptors and mediate membrane fusion and virus entry into host cells(PMID: 32221306). Each monomer of trimeric S protein is about 180 kDa, and contains two subunits, S1 and S2,S1 recognizes and binds to host receptors, and subsequent conformational changes in S2 facilitate fusion between the viral envelope and the host cell membrane(PMID: 19198616). Although the amino acid sequences of the S-glycoprotein were found to be different between the various HCoV, the structures showed high similarity, but the best 3D structural overlap shared by SARS-CoV and SARS-CoV-2, consistent with the shared ACE2 predicted receptor (PMID: 32522207). The spike protein of CoVs can be a target for vaccine and therapeutic development (PMID: 19198616).
Lyophilized format of this product is available.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SARS-CoV-2 S protein (RBD, 319-541 aa) antibody 67758-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Mater A Universal Therapeutic Vaccine Leveraging Autologous Pre-Existing Immunity to Eliminate in Situ Uniformly Engineered Heterogeneous Tumor Cells | ||
Acta Pharm Sin B A novel heterologous receptor-binding domain dodecamer universal mRNA vaccine against SARS-CoV-2 variants | ||
Sci Adv Manganese-coordinated mRNA vaccines with enhanced mRNA expression and immunogenicity induce robust immune responses against SARS-CoV-2 variants | ||
Adv Healthc Mater Black Phosphorus Nanosheets Assist Nanoerythrosomes for Efficient Mrna Vaccine Delivery and Immune Activation | ||
ACS Sens A Field-Deployable Microfluidic CNT-FET Platform for Direct Monitoring of Multiplexed Respiratory Viruses in Environmental Waters. | ||
Front Immunol B cell receptor repertoire analysis from autopsy samples of COVID-19 patients |
