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验证数据展示

  • WB analysis of Recombinant protein using 67758-1-Ig (same clone as 67758-1-PBS)

    WB analysis of Recombinant protein using 67758-1-Ig (same clone as 67758-1-PBS)

    Recombinant spike protein (RBD domain) were subjected to SDS PAGE followed by western blot with 67758-1-lg (SARS-CoV-2 Spike Glycoprotein (319-541 aa) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67758-1-PBS in a different storage buffer formulation.

  • Neutralization experiment of SARS-CoV-2 S protein (RBD, 319-541 aa) using 67758-1-Ig (same clone as 67758-1-PBS)

    Neutralization experiment of SARS-CoV-2 S protein (RBD, 319-541 aa) using 67758-1-Ig (same clone as 67758-1-PBS)

    Surrogate virus neutralization test of SARS-CoV-2 Spike Glycoprotein (319-541 aa) monoclonal antibody 67758-1-Ig using commercial kit. Briefly, RBD protein has been pre-coated on microplate, HRP labeled ACE2 protein and serial dose of 67758-1-Ig are added to the plate simultaneously and incubated for 1 hour at 37°C. The plate was then washed and signal was developed by adding chromogenic substrate followed by stop buffer. Signal strength was monitored at absorbance of 450nm. The IC50 is around 20 μg/mL. Please note that different IC50 will be obtained if different assays are used. Lyophilized format of this product is available. This data was developed using the same antibody clone with 67758-1-PBS in a different storage buffer formulation.

Virus SARS-CoV-2 S protein (RBD, 319-541 aa) Monoclonal antibody, PBS Only

SARS-CoV-2 S protein (RBD, 319-541 aa) Monoclonal Antibody for WB, Neutralization, Indirect ELISA
Cat No. 67758-1-PBS

产品说明书

CloneNo. 1H3E9

宿主/亚型

Mouse / IgG1

种属反应性

Virus

应用

WB, Neutralization, Indirect ELISA

别名

COVID-19 RBD, RBD, SARS-CoV-2 Spike Glycoprotein (319-541 aa), spike protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

67758-1-PBS targets SARS-CoV-2 S protein (RBD, 319-541 aa) in WB, Neutralization, Indirect ELISA applications and shows reactivity with Virus samples.

经测试应用 WB, Neutralization, Indirect ELISA Application Description
经测试反应性 Virus
免疫原 SARS-CoV-2 S protein (RBD, 319-541 aa) fusion protein Ag30688 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 SARS-CoV-2 Spike Protein
别名 COVID-19 RBD, RBD, SARS-CoV-2 Spike Glycoprotein (319-541 aa), spike protein
计算分子量 141 kDa
GenBank蛋白编号NC_045512
基因名称 SARS-COV-2
Gene ID (NCBI) 43740568
RRIDAB_2918526
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Coronaviruses (CoVs) infect human and animals and cause varieties of diseases, including respiratory, enteric, renal, and neurological diseases. CoV uses its spike protein to recognize ACE2 as its receptors and mediate membrane fusion and virus entry into host cells(PMID: 32221306). Each monomer of trimeric S protein is about 180 kDa, and contains two subunits, S1 and S2,S1 recognizes and binds to host receptors, and subsequent conformational changes in S2 facilitate fusion between the viral envelope and the host cell membrane(PMID: 19198616). Although the amino acid sequences of the S-glycoprotein were found to be different between the various HCoV, the structures showed high similarity, but the best 3D structural overlap shared by SARS-CoV and SARS-CoV-2, consistent with the shared ACE2 predicted receptor (PMID: 32522207). The spike protein of CoVs can be a target for vaccine and therapeutic development (PMID: 19198616).

Lyophilized format of this product is available.