验证数据展示
经过测试的应用
| Positive WB detected in | Ag30688 |
| Positive Neutralization detected in | Recombinant protein |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| NEUTRALIZATION | NEUTRALIZATION : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 9 publications below |
产品信息
67758-1-Ig targets SARS-CoV-2 S protein (RBD, 319-541 aa) in WB, Neutralization, ELISA applications and shows reactivity with Virus samples.
| 经测试应用 | WB, ELISA, Neutralization Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Virus |
| 文献引用反应性 | human, mouse |
| 免疫原 | SARS-CoV-2 S protein (RBD, 319-541 aa) fusion protein Ag30688 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | SARS-CoV-2 Spike Protein |
| 别名 | COVID-19 RBD, RBD, SARS-CoV-2 Spike Glycoprotein (319-541 aa), spike protein |
| 计算分子量 | 141 kDa |
| GenBank蛋白编号 | NC_045512 |
| 基因名称 | SARS-COV-2 |
| Gene ID (NCBI) | 43740568 |
| RRID | AB_2918526 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Coronaviruses (CoVs) infect human and animals and cause varieties of diseases, including respiratory, enteric, renal, and neurological diseases. CoV uses its spike protein to recognize ACE2 as its receptors and mediate membrane fusion and virus entry into host cells(PMID: 32221306). Each monomer of trimeric S protein is about 180 kDa, and contains two subunits, S1 and S2,S1 recognizes and binds to host receptors, and subsequent conformational changes in S2 facilitate fusion between the viral envelope and the host cell membrane(PMID: 19198616). Although the amino acid sequences of the S-glycoprotein were found to be different between the various HCoV, the structures showed high similarity, but the best 3D structural overlap shared by SARS-CoV and SARS-CoV-2, consistent with the shared ACE2 predicted receptor (PMID: 32522207). The spike protein of CoVs can be a target for vaccine and therapeutic development (PMID: 19198616).
Lyophilized format of this product is available.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SARS-CoV-2 S protein (RBD, 319-541 aa) antibody 67758-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Acta Pharm Sin B A novel heterologous receptor-binding domain dodecamer universal mRNA vaccine against SARS-CoV-2 variants | ||
J Med Virol A novel three-plasmid packaging system for chimeric SFV/SIN VRPs derived from Semliki Forest virus and Sindbis virus as a candidate gene delivery vector | ||
bioRxiv Novel sensor-integrated proteome on chip (SPOC) platform with thousands of folded proteins on a 1.5 sq-cm biosensor chip to enable high-throughput real-time label-free screening for kinetic analysis | ||
Virol J LINC01002 functions as a ceRNA to regulate FRMD8 by sponging miR-4324 for the development of COVID-19 | ||
Int J Mol Sci SARS-CoV-2 Rapidly Infects Peripheral Sensory and Autonomic Neurons, Contributing to Central Nervous System Neuroinvasion before Viremia |