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验证数据展示

  • WB analysis of Recombinant protein using 80027-1-RR

    WB analysis of Recombinant protein using 80027-1-RR

    E.coli expressed SARS-CoV-2 Nucleocapsid Phosphoprotein (Cat.NO. Ag30676) was subjected to SDS-PAGE followed by western blot with 80027-1-RR and 80026-1-RR at various work concentration.

  • WB analysis of Recombinant protein using 80027-1-RR

    WB analysis of Recombinant protein using 80027-1-RR

    Eukaryotic expressed SARS-CoV-2 Nucleocapsid Phosphoprotein was subjected to SDS-PAGE followed by western blot with 80027-1-RR and 80026-1-RR at various work concentration.

  • ELISA experiment of Recombinant protein using 80027-1-RR

    ELISA experiment of Recombinant protein using 80027-1-RR

    Indirect ELISA was carried out by coating eukaryotic expressed N protein at 70 ng/well followed by blocking and adding serial diluted primary antibody 80026-1-RR and 80027-1-RR respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

  • ELISA experiment of Recombinant protein using 80027-1-RR

    ELISA experiment of Recombinant protein using 80027-1-RR

    Sandwich ELISA was carried out by coating 80027-1-RR at 80 ng/well followed by blocking and adding different concentration of eukaryotic expressed N protein (0.5-1000 ng/mL). HRP-conjugated80026-1-RR was used at 1 μg/mL for detection. Signal was developed with TMB and stopped by H2SO4 . Signal strength was measured by absorbance at 450 nm.

  • Affinity and Kinetic Characterization of 80027-1-RR

    Affinity and Kinetic Characterization of 80027-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80027-1-RR against SARS-CoV-2 Nucleocapsid Phosphoprotein were performed. The affinity constant is 1.02 nM.

SARS-CoV-2 Nucleocapsid Phosphoprotein Recombinant antibody

SARS-CoV-2 Nucleocapsid Phosphoprotein Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 80027-1-RR

产品说明书

CloneNo. 8C20

宿主/亚型

Rabbit / IgG

种属反应性

virus, recombinant protein

应用

WB, ELISA

别名

nucleocapsid phosphoprotein, sars cov 2, 8C20

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inEukaryotic nucleocapsid phosphoprotein, Recombinant protein
Positive ELISA detected inRecombinant protein

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Enzyme-linked Immunosorbent Assay (ELISA)ELISA : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80027-1-RR targets SARS-CoV-2 Nucleocapsid Phosphoprotein in WB, ELISA applications and shows reactivity with virus, recombinant protein samples.

经测试应用 WB, ELISA Application Description
经测试反应性 virus, recombinant protein
免疫原 SARS-CoV-2 Nucleocapsid Phosphoprotein fusion protein Ag30676 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 COVID-19 N Protein
别名 nucleocapsid phosphoprotein, sars cov 2, 8C20
GenBank蛋白编号NC_045512
基因名称 COVID-19 N Protein
Gene ID (NCBI) 43740575
RRIDAB_2882945
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The nucleocapsid (N) protein has multiple functions including formation of nucleocapsids, signal transduction virus budding, RNA replication, and mRNA transcription. N protein is an important antigen for coronavirus, and it is normally highly conserved, with a molecular weight of about 50 kDa. it can be used as a marker in diagnostic assays due to its high immunogenicity (PMID: 32416961, PMID: 32235387). A sandwich ELISA for COVID-19 N Protein can be assembled by using 80027-1-RR as capture antibody and conjugated 80026-1-RR for detection.

实验方案

Product Specific Protocols
WB protocol for SARS-CoV-2 Nucleocapsid Phosphoprotein antibody 80027-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols