E.coli expressed SARS-CoV-2 Nucleocapsid Phosphoprotein (Cat.NO. Ag30676) was subjected to SDS-PAGE followed by western blot with 80027-1-RR and 80026-1-RR at various work concentration. This data was developed using the same antibody clone with 80027-1-PBS in a different storage buffer formulation.

Eukaryotic expressed SARS-CoV-2 Nucleocapsid Phosphoprotein was subjected to SDS-PAGE followed by western blot with 80027-1-RR and 80026-1-RR at various work concentration. This data was developed using the same antibody clone with 80027-1-PBS in a different storage buffer formulation.

Indirect ELISA was carried out by coating eukaryotic expressed N protein at 70 ng/well followed by blocking and adding serial diluted primary antibody 80026-1-RR and 80027-1-RR respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm. This data was developed using the same antibody clone with 80027-1-PBS in a different storage buffer formulation.

Sandwich ELISA was carried out by coating 80027-1-RR at 80 ng/well followed by blocking and adding different concentration of eukaryotic expressed N protein (0.5-1000 ng/mL). HRP-conjugated80026-1-RR was used at 1 μg/mL for detection. Signal was developed with TMB and stopped by H2SO4 . Signal strength was measured by absorbance at 450 nm. This data was developed using the same antibody clone with 80027-1-PBS in a different storage buffer formulation.

Biolayer interferometry (BLl) kinetic assays of 80027-1-RR against SARS-CoV-2 Nucleocapsid Phosphoprotein were performed. The affinity constant is 1.02 nM.