CoraLite® Plus 488-conjugated BOULE Polyclonal antibody
BOULE Polyclonal Antibody for IF-P, IF-Fro
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF-P, IF-Fro
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse testis tissue |
| Positive IF-Fro detected in | mouse testis tissue |
For other applications, we recommend the unconjugated version of this antibody, 13720-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-13720 targets BOULE in IF-P, IF-Fro applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF-P, IF-Fro Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | BOULE fusion protein Ag4662 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | bol, boule-like (Drosophila) |
| Synonyms | BOLL, Protein boule-like |
| Calculated Molecular Weight | 283 aa, 31 kDa |
| GenBank Accession Number | BC033674 |
| Gene Symbol | BOULE |
| Gene ID (NCBI) | 66037 |
| RRID | AB_2919076 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N9W6 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BOULE, also called BOLL, belongs to the RRM DAZ family. BOULE has been identified as being involved in regulating the development and differentiation of germ cells and playing an essential role in infertility (PMID: 31683986). BOULE is specifically expressed in testis, but not in early embryos, primordial germ cells, and spermatogonial cells. It is first expressed in the cytoplasm of spermatocytes and then persists through meiosis. BOULE has 5 isoforms with the molecular mass of 19 and 31-38 kDa. This antibody detects two bands in testis, the lower band of ~40 kDa in size corresponds to the predicted endogenous BOULE protein, while the upper band may be an isoform or posttranslationally modified version of the BOULE protein (PMID: 27632217).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 BOULE antibody CL488-13720 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
