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验证数据展示

  • IF Staining of mouse testis using CL488-13720

    IF Staining of mouse testis using CL488-13720

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 488 BOULE antibody (CL488-13720) at dilution of 1:400, CoraLite®594 DAZL antibody (CL594-12633, red), CoraLite® Plus 647 TNP1 antibody (CL647-17178, Magenta).

  • IF Staining of mouse testis using CL488-13720

    IF Staining of mouse testis using CL488-13720

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 488 BOULE antibody (CL488-13720) at dilution of 1:400, CoraLite®594 DAZL antibody (CL594-12633, red), CoraLite® Plus 647 TNP1 antibody (CL647-17178, Magenta).

  • IF Staining of mouse testis using CL488-13720

    IF Staining of mouse testis using CL488-13720

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse testis tissue using CoraLite® Plus 488 CL488-13720 (BOULE antibody) at dilution of 1:100, CoraLite®555 CL555-17178 (TNP1 antibody, orange) and 67130-1-Ig (STAR antibody, Magenta).

CoraLite® Plus 488-conjugated BOULE Polyclonal antibody

BOULE Polyclonal Antibody for IF-P, IF-Fro
Cat No. CL488-13720

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF-P, IF-Fro

别名

BOLL, Protein boule-like

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse testis tissue
Positive IF-Fro detected inmouse testis tissue
Planning an IHC experiment? We recommend our IHCeasy BOLL/BOULE Ready-To-Use IHC Kit. BOLL/BOULE primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 13720-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)-FROIF-FRO : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-13720 targets BOULE in IF-P, IF-Fro applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF-P, IF-Fro Application Description
经测试反应性 human, mouse, rat
免疫原 BOULE fusion protein Ag4662 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 bol, boule-like (Drosophila)
别名 BOLL, Protein boule-like
计算分子量 283 aa, 31 kDa
GenBank蛋白编号BC033674
基因名称 BOULE
Gene ID (NCBI) 66037
RRIDAB_2919076
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N9W6
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BOULE, also called BOLL, belongs to the RRM DAZ family. BOULE has been identified as being involved in regulating the development and differentiation of germ cells and playing an essential role in infertility (PMID: 31683986). BOULE is specifically expressed in testis, but not in early embryos, primordial germ cells, and spermatogonial cells. It is first expressed in the cytoplasm of spermatocytes and then persists through meiosis. BOULE has 5 isoforms with the molecular mass of 19 and 31-38 kDa. This antibody detects two bands in testis, the lower band of ~40 kDa in size corresponds to the predicted endogenous BOULE protein, while the upper band may be an isoform or posttranslationally modified version of the BOULE protein (PMID: 27632217).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 BOULE antibody CL488-13720Download protocol
Standard Protocols
Click here to view our Standard Protocols