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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated ACLY Polyclonal antibody

ACLY Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat no : CL647-15421

Print datasheet

Synonyms

ATP citrate lyase, ACL, pro-S-, EC:2.3.3.8, ATP-citrate synthase

验证数据展示

  • FC experiment of HepG2 using CL647-15421

    FC experiment of HepG2 using CL647-15421

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human ACLY (CL647-15421) (red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ACLY Ready-To-Use IHC Kit. ACLY primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 15421-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-15421 targets ACLY in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen ACLY fusion protein Ag7709 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATP citrate lyase
Synonyms ATP citrate lyase, ACL, pro-S-, EC:2.3.3.8, ATP-citrate synthase
Calculated Molecular Weight 121 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC006195
Gene Symbol ACLY
Gene ID (NCBI) 47
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP53396
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACLY(ATP-citrate synthase) is also named as ACL. It is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA. ACLY serves as not only a target in oxygenated cells for suppression of lipid synthesis and histone acetylation, but also as a susceptible target in hypoxic cells to restore inhibition of glycolysis. In nonsmall cell lung carcinoma and hepatocellular carcinoma, ACLY is overexpressed compared with normal parenchyma suggesting that ACLY may represent a common target among highly malignant tumors(PMID:19795461). This protein has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 ACLY antibody CL647-15421Download protocol
Standard Protocols
Click here to view our Standard Protocols