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验证数据展示

  • WB analysis using 23660-1-AP

    WB analysis using 23660-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23660-1-AP (alpha Actin antibody) at dilution of 1:150000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 23660-1-AP

    IHC staining of human colon using 23660-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon slide using 23660-1-AP (alpha Actin antibody at dilution of 1:50.

  • IHC staining of human colon using 23660-1-AP

    IHC staining of human colon using 23660-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon slide using 23660-1-AP (alpha Actin antibody at dilution of 1:50.

  • IHC staining of mouse skeletal muscle using 23660-1-AP

    IHC staining of mouse skeletal muscle using 23660-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 23660-1-AP (Alpha Actin antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of mouse skeletal muscle using 23660-1-AP

    IP experiment of mouse skeletal muscle using 23660-1-AP

    IP result of anti-Alpha Actin (IP:23660-1-AP, 4ug; Detection:23660-1-AP 1:4000) with mouse skeletal muscle tissue lysate 2200ug.

  • IHC staining of mouse skeletal muscle using 23660-1-AP

    IHC staining of mouse skeletal muscle using 23660-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 23660-1-AP (Alpha Actin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse heart using 23660-1-AP

    IF Staining of mouse heart using 23660-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Alpha Actin antibody (23660-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse heart using 23660-1-AP

    IF Staining of mouse heart using 23660-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using Alpha Actin antibody (23660-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse skeletal muscle using 23660-1-AP

    IF Staining of mouse skeletal muscle using 23660-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using Alpha Actin antibody (23660-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of AC16 using 23660-1-AP

    IF Staining of AC16 using 23660-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed AC16 cells using Alpha Actin antibody (23660-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • IF Staining of H9C2 using 23660-1-AP

    IF Staining of H9C2 using 23660-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed H9C2 cells using Alpha Actin antibody (23660-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • FC experiment of C2C12 using 23660-1-AP

    FC experiment of C2C12 using 23660-1-AP

    1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human Alpha Actin (23660-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Alpha Actin Polyclonal antibody

Alpha Actin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA
Cat No. 23660-1-AP

产品说明书

引用文献(225)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA and More (1)

别名

ACTA1, α actin, ACTA, ACTA1 skeletal Muscle Actin, Actin alpha skeletal muscle

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue, human colon tissue, human heart tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse heart tissue, mouse skeletal muscle tissue
Positive IF/ICC detected inAC16 cells, H9C2 cells
Positive FC (Intra) detected inC2C12 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23660-1-AP targets Alpha Actin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, pig
免疫原

CatNo: Ag19151

Product name: Recombinant human ACTA1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-377 aa of BC012597

Sequence: MCDEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHRKCF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 actin, alpha 1, skeletal muscle
别名 ACTA1, α actin, ACTA, ACTA1 skeletal Muscle Actin, Actin alpha skeletal muscle
计算分子量 42 kDa
观测分子量 39-42 kDaa
GenBank蛋白编号BC012597
基因名称 ACTA1
Gene ID (NCBI) 58
RRIDAB_2879307
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen Affinity purified
UNIPROT IDP68133
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for Alpha Actin antibody 23660-1-APDownload protocol
IF protocol for Alpha Actin antibody 23660-1-APDownload protocol
IHC protocol for Alpha Actin antibody 23660-1-APDownload protocol
IP protocol for Alpha Actin antibody 23660-1-APDownload protocol
WB protocol for Alpha Actin antibody 23660-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

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