验证数据展示
经过测试的应用
| Positive WB detected in | mouse skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human colon tissue, human heart tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
| Positive IF/ICC detected in | H9C2 cells |
| Positive FC (Intra) detected in | C2C12 cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
23660-1-AP targets Alpha Actin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig |
| 免疫原 | Alpha Actin fusion protein Ag19151 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | actin, alpha 1, skeletal muscle |
| 别名 | ACTA1, α actin, ACTA, Actin, alpha skeletal muscle, intermediate form, Actin, alpha skeletal muscle |
| 计算分子量 | 42 kDa |
| 观测分子量 | 39-42 kDaa |
| GenBank蛋白编号 | BC012597 |
| 基因名称 | ACTA1 |
| Gene ID (NCBI) | 58 |
| RRID | AB_2879307 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | P68133 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Alpha Actin antibody 23660-1-AP | Download protocol |
| IHC protocol for Alpha Actin antibody 23660-1-AP | Download protocol |
| IF protocol for Alpha Actin antibody 23660-1-AP | Download protocol |
| IP protocol for Alpha Actin antibody 23660-1-AP | Download protocol |
| FC protocol for Alpha Actin antibody 23660-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Overcoming therapeutic resistance in oncolytic herpes virotherapy by targeting IGF2BP3-induced NETosis in malignant glioma | ||
J Exp Med Tumor cell-intrinsic Piezo2 drives radioresistance by impairing CD8+ T cell stemness maintenance | ||
Acta Pharm Sin B A novel SIRT6 activator ameliorates neuroinflammation and ischemic brain injury via EZH2/FOXC1 axis. | ||
Transl Res Different roles of BAG3 in cardiac physiological hypertrophy and pathological remodeling. | ||
Redox Biol Mitochondrial dysfunction by TFAM depletion disrupts self-renewal and lineage differentiation of human PSCs by affecting cell proliferation and YAP response. |
