验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse heart tissue |
| Positive IF/ICC detected in | H9C2 cells, C2C12 cells |
For other applications, we recommend the unconjugated version of this antibody, 23660-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-23660 targets Alpha Actin in IF/ICC, IF-P applications and shows reactivity with mouse, rat samples.
| Tested Applications | IF/ICC, IF-P Application Description |
| Tested Reactivity | mouse, rat |
| Immunogen | Alpha Actin fusion protein Ag19151 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | actin, alpha 1, skeletal muscle |
| Synonyms | ACTA1, α actin, ACTA, Actin, alpha skeletal muscle, intermediate form, Actin, alpha skeletal muscle |
| Calculated Molecular Weight | 42 kDa |
| Observed Molecular Weight | 40-42 kDa |
| GenBank Accession Number | BC012597 |
| Gene Symbol | ACTA1 |
| Gene ID (NCBI) | 58 |
| RRID | AB_3084076 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P68133 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Alpha Actin antibody CL488-23660 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |