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验证数据展示

  • IF Staining of mouse heart using CL488-23660

    IF Staining of mouse heart using CL488-23660

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using CoraLite® Plus 488 Alpha Actin antibody (CL488-23660) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of H9C2 using CL488-23660

    IF Staining of H9C2 using CL488-23660

    Immunofluorescent analysis of (-20°C Methanol) fixed H9C2 cells using CoraLite® Plus 488 Alpha Actin antibody (CL488-23660) at dilution of 1:200.

  • IF Staining of C2C12 using CL488-23660

    IF Staining of C2C12 using CL488-23660

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using CoraLite® Plus 488 Alpha Actin antibody (CL488-23660) at dilution of 1:200.

CoraLite® Plus 488-conjugated Alpha Actin Polyclonal antibody

Alpha Actin Polyclonal Antibody for IF/ICC, IF-P
Cat No. CL488-23660

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

IF/ICC, IF-P

别名

ACTA1, α actin, ACTA, Actin, alpha skeletal muscle, intermediate form, Actin, alpha skeletal muscle

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse heart tissue
Positive IF/ICC detected inH9C2 cells, C2C12 cells
For other applications, we recommend the unconjugated version of this antibody, 23660-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-23660 targets Alpha Actin in IF/ICC, IF-P applications and shows reactivity with mouse, rat samples.

经测试应用 IF/ICC, IF-P Application Description
经测试反应性 mouse, rat
免疫原 Alpha Actin fusion protein Ag19151 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 actin, alpha 1, skeletal muscle
别名 ACTA1, α actin, ACTA, Actin, alpha skeletal muscle, intermediate form, Actin, alpha skeletal muscle
计算分子量 42 kDa
观测分子量 40-42 kDa
GenBank蛋白编号BC012597
基因名称 ACTA1
Gene ID (NCBI) 58
RRIDAB_3084076
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP68133
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Alpha Actin antibody CL488-23660Download protocol
Standard Protocols
Click here to view our Standard Protocols