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CoraLite® Plus 488-conjugated Alpha Actin Polyclonal antibody

Alpha Actin Polyclonal Antibody for IF/ICC, IF-P

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

IF/ICC, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-23660

Print datasheet

Synonyms

ACTA1, α actin, ACTA, Actin, alpha skeletal muscle, intermediate form, Actin, alpha skeletal muscle

验证数据展示

  • IF Staining of mouse heart using CL488-23660

    IF Staining of mouse heart using CL488-23660

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using CoraLite® Plus 488 Alpha Actin antibody (CL488-23660) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of H9C2 using CL488-23660

    IF Staining of H9C2 using CL488-23660

    Immunofluorescent analysis of (-20°C Methanol) fixed H9C2 cells using CoraLite® Plus 488 Alpha Actin antibody (CL488-23660) at dilution of 1:200.

  • IF Staining of C2C12 using CL488-23660

    IF Staining of C2C12 using CL488-23660

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using CoraLite® Plus 488 Alpha Actin antibody (CL488-23660) at dilution of 1:200.

经过测试的应用

Positive IF-P detected inmouse heart tissue
Positive IF/ICC detected inH9C2 cells, C2C12 cells
For other applications, we recommend the unconjugated version of this antibody, 23660-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-23660 targets Alpha Actin in IF/ICC, IF-P applications and shows reactivity with mouse, rat samples.

Tested Applications IF/ICC, IF-P Application Description
Tested Reactivity mouse, rat
Immunogen Alpha Actin fusion protein Ag19151 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name actin, alpha 1, skeletal muscle
Synonyms ACTA1, α actin, ACTA, Actin, alpha skeletal muscle, intermediate form, Actin, alpha skeletal muscle
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 40-42 kDa
GenBank Accession NumberBC012597
Gene Symbol ACTA1
Gene ID (NCBI) 58
RRIDAB_3084076
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP68133
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Alpha Actin antibody CL488-23660Download protocol
Standard Protocols
Click here to view our Standard Protocols