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ACTC1-specific Monoclonal antibody, PBS Only

ACTC1-specific Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F2B9

Cat no : 66125-1-PBS

Print datasheet

Synonyms

ACTC1, Alpha actin, actin, ACTC1 cardiac muscle 1, ACTC 1

验证数据展示

  • WB analysis using 66125-1-Ig (same clone as 66125-1-PBS)

    WB analysis using 66125-1-Ig (same clone as 66125-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66125-1-Ig (ACTC1-specific antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart and human skeletal muscle tissue using 66125-1-Ig (same clone as 66125-1-PBS)

    IHC staining of human heart and human skeletal muscle tissue using 66125-1-Ig (same clone as 66125-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart and human skeletal muscle using 66125-1-Ig (ACTC1 specific antibody) at dilution 1:500. (under 40x lens). This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ACTC1-specific antibody included
  • IHC staining of human heart using 66125-1-Ig (same clone as 66125-1-PBS)

    IHC staining of human heart using 66125-1-Ig (same clone as 66125-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 66125-1-Ig(ACTC1-specific antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ACTC1-specific antibody included
  • IHC staining of human heart using 66125-1-Ig (same clone as 66125-1-PBS)

    IHC staining of human heart using 66125-1-Ig (same clone as 66125-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 66125-1-Ig(ACTC1-specific antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ACTC1-specific antibody included
  • WB analysis of human heart using 66125-1-Ig (same clone as 66125-1-PBS)

    WB analysis of human heart using 66125-1-Ig (same clone as 66125-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 66125-1-Ig (ACTC1-specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

  • FC experiment of C2C12 using 66125-1-Ig (same clone as 66125-1-PBS)

    FC experiment of C2C12 using 66125-1-Ig (same clone as 66125-1-PBS)

    1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human ACTC1-specific (66125-1-Ig, Clone:1F2B9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 66125-1-Ig (same clone as 66125-1-PBS)

    IF Staining of mouse heart using 66125-1-Ig (same clone as 66125-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using ACTC1-specific antibody (66125-1-Ig, Clone: 1F2B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy ACTC1-specific Ready-To-Use IHC Kit. ACTC1-specific primary antibody included.

产品信息

66125-1-PBS targets ACTC1-specific in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF-P, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name actin, alpha, cardiac muscle 1
Synonyms ACTC1, Alpha actin, actin, ACTC1 cardiac muscle 1, ACTC 1
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberNM_005159
Gene Symbol ACTC1
Gene ID (NCBI) 70
RRIDAB_2881524
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP68032
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTC1 gene encodes cardiac muscle alpha-actin, the predominant actin isoform in adult heart, which interacts with a variety of proteins to produce the force for muscle contraction. This antibody is specific to the ACTC1. It does not cross-react with other actin isoforms.