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ACTC1-specific Monoclonal antibody, PBS Only

ACTC1-specific Monoclonal Antibody for WB,Indirect ELISA,IHC,IF,FC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, pig, rat

Applications

WB,Indirect ELISA,IHC,IF,FC

Conjugate

Unconjugated

CloneNo.

1F2B9

Cat No : 66125-1-PBS

Print datasheet

Synonyms

ACTC, ACTC1, ACTC1 cardiac muscle 1, ACTC1 specific, ACTC1-specific, Actin cardiac muscle specific, Actin, alpha cardiac muscle 1, actin, alpha, cardiac muscle 1, Alpha cardiac actin, CMD1R, CMH11

验证数据展示

  • WB analysis using 66125-1-Ig (same clone as 66125-1-PBS)

    WB analysis using 66125-1-Ig (same clone as 66125-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66125-1-Ig (ACTC1-specific antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart and human skeletal muscle tissue using 66125-1-Ig (same clone as 66125-1-PBS)

    IHC staining of human heart and human skeletal muscle tissue using 66125-1-Ig (same clone as 66125-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart and human skeletal muscle using 66125-1-Ig (ACTC1 specific antibody) at dilution 1:500. (under 40x lens). This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ACTC1-specific antibody included
  • IHC staining of human heart using 66125-1-Ig (same clone as 66125-1-PBS)

    IHC staining of human heart using 66125-1-Ig (same clone as 66125-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 66125-1-Ig(ACTC1-specific antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ACTC1-specific antibody included
  • IHC staining of human heart using 66125-1-Ig (same clone as 66125-1-PBS)

    IHC staining of human heart using 66125-1-Ig (same clone as 66125-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 66125-1-Ig(ACTC1-specific antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ACTC1-specific antibody included
  • WB analysis of human heart using 66125-1-Ig (same clone as 66125-1-PBS)

    WB analysis of human heart using 66125-1-Ig (same clone as 66125-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 66125-1-Ig (ACTC1-specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

  • FC experiment of C2C12 using 66125-1-Ig (same clone as 66125-1-PBS)

    FC experiment of C2C12 using 66125-1-Ig (same clone as 66125-1-PBS)

    1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human ACTC1-specific (66125-1-Ig, Clone:1F2B9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 66125-1-Ig (same clone as 66125-1-PBS)

    IF Staining of mouse heart using 66125-1-Ig (same clone as 66125-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using ACTC1-specific antibody (66125-1-Ig, Clone: 1F2B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66125-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy ACTC1-specific Ready-To-Use IHC Kit. ACTC1-specific primary antibody included.

产品信息

66125-1-PBS targets ACTC1-specific in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with human, mouse, pig, rat samples.

Tested Applications WB,Indirect ELISA,IHC,IF,FC Application Description
Tested Reactivity human, mouse, pig, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name actin, alpha, cardiac muscle 1
Synonyms ACTC, ACTC1, ACTC1 cardiac muscle 1, ACTC1 specific, ACTC1-specific, Actin cardiac muscle specific, Actin, alpha cardiac muscle 1, actin, alpha, cardiac muscle 1, Alpha cardiac actin, CMD1R, CMH11
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberNM_005159
Gene Symbol ACTC1
Gene ID (NCBI) 70
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP68032
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTC1 gene encodes cardiac muscle alpha-actin, the predominant actin isoform in adult heart, which interacts with a variety of proteins to produce the force for muscle contraction. This antibody is specific to the ACTC1. It does not cross-react with other actin isoforms.