验证数据展示
经过测试的应用
| Positive WB detected in | human heart tissue, pig heart tissue, rat heart tissue, mouse heart tissue |
| Positive IHC detected in | human heart tissue, human heart and human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
| Positive FC (Intra) detected in | C2C12 cells |
Planning an IHC experiment? We recommend our IHCeasy ACTC1-specific Ready-To-Use IHC Kit. ACTC1-specific primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2500-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66125-1-Ig targets ACTC1-specific in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Applications | WB, IHC, IF-P, FC (Intra), ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat, rabbit |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | actin, alpha, cardiac muscle 1 |
| Synonyms | ACTC1, Alpha actin, actin, ACTC1 cardiac muscle 1, ACTC 1 |
| Calculated Molecular Weight | 42 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | NM_005159 |
| Gene Symbol | ACTC1 |
| Gene ID (NCBI) | 70 |
| RRID | AB_2881524 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P68032 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTC1 gene encodes cardiac muscle alpha-actin, the predominant actin isoform in adult heart, which interacts with a variety of proteins to produce the force for muscle contraction. This antibody is specific to the ACTC1. It does not cross-react with other actin isoforms.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ACTC1-specific antibody 66125-1-Ig | Download protocol |
| IHC protocol for ACTC1-specific antibody 66125-1-Ig | Download protocol |
| IF protocol for ACTC1-specific antibody 66125-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Life Sci Cadmium exposure induces a sex-dependent decline in left ventricular cardiac function | ||
J Am Heart Assoc Postinfarction Hearts Are Protected by Premature Senescent Cardiomyocytes Via GATA 4-Dependent CCN 1 Secretion. | ||
Front Mol Biosci Co-Overexpression of GRK5/ACTC1 Correlates With the Clinical Parameters and Poor Prognosis of Epithelial Ovarian Cancer. | ||
Immunol Res The influences of α-hemolytic Streptococcus on class switching and complement activation of human tonsillar cells in IgA nephropathy. | ||
J Clin Invest Frameshift variants in C10orf71 cause dilated cardiomyopathy in human, mouse, and organoid models |
