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验证数据展示

  • IF Staining of mouse heart using CL488-66125

    IF Staining of mouse heart using CL488-66125

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CL488-66125 (ACTC1-specific antibody) at dilution of 1:100.

  • IF Staining of mouse heart using CL488-66125

    IF Staining of mouse heart using CL488-66125

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CL488-66125 (ACTC1-specific antibody) at dilution of 1:100.

CoraLite® Plus 488-conjugated ACTC1-specific Monoclonal antibody

ACTC1-specific Monoclonal Antibody for IF-P
Cat No. CL488-66125

产品说明书

CloneNo. 1F2B9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, pig, rat

应用

IF-P

别名

ACTC, ACTC1, ACTC1 cardiac muscle 1, ACTC1-specific, Actin, alpha cardiac muscle 1, actin, alpha, cardiac muscle 1, Alpha cardiac actin, CMD1R, CMH11

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse heart tissue
Planning an IHC experiment? We recommend our IHCeasy ACTC1-specific Ready-To-Use IHC Kit. ACTC1-specific primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66125-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66125 targets ACTC1-specific in IF-P applications and shows reactivity with human, mouse, pig, rat samples.

经测试应用 IF-P Application Description
经测试反应性 human, mouse, pig, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 actin, alpha, cardiac muscle 1
别名 ACTC, ACTC1, ACTC1 cardiac muscle 1, ACTC1-specific, Actin, alpha cardiac muscle 1, actin, alpha, cardiac muscle 1, Alpha cardiac actin, CMD1R, CMH11
计算分子量 42 kDa
GenBank蛋白编号NM_005159
基因名称 ACTC1
Gene ID (NCBI) 70
RRIDAB_2883266
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP68032
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTC1 gene encodes cardiac muscle alpha-actin, the predominant actin isoform in adult heart, which interacts with a variety of proteins to produce the force for muscle contraction. This antibody is specific to the ACTC1. It does not cross-react with other actin isoforms. The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ACTC1-specific antibody CL488-66125Download protocol
Standard Protocols
Click here to view our Standard Protocols