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  • Featured Product
  • KD/KO Validated

AGO2 Monoclonal antibody, PBS Only

AGO2 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1G2H12

Cat no : 67934-1-PBS

Print datasheet

Synonyms

EIF2C2, EC:3.1.26.n2, Argonaute2, Argonaute RISC catalytic component 2, Argonaute 2

验证数据展示

  • WB analysis using 67934-1-Ig (same clone as 67934-1-PBS)

    WB analysis using 67934-1-Ig (same clone as 67934-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67934-1-Ig (AGO2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67934-1-PBS in a different storage buffer formulation.

  • WB analysis using 67934-1-Ig (same clone as 67934-1-PBS)

    WB analysis using 67934-1-Ig (same clone as 67934-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67934-1-Ig (AGO2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67934-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 67934-1-Ig (same clone as 67934-1-PBS)

    WB analysis of HepG2 using 67934-1-Ig (same clone as 67934-1-PBS)

    WB result of AGO2 antibody (67934-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AGO2 transfected HepG2 cells. This data was developed using the same antibody clone with 67934-1-PBS in a different storage buffer formulation.

  • IHC staining of human urothelial carcinoma using 67934-1-Ig (same clone as 67934-1-PBS)

    IHC staining of human urothelial carcinoma using 67934-1-Ig (same clone as 67934-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 67934-1-Ig (AGO2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67934-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AGO2/EIF2C2 antibody included
  • IHC staining of human pancreas cancer using 67934-1-Ig (same clone as 67934-1-PBS)

    IHC staining of human pancreas cancer using 67934-1-Ig (same clone as 67934-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67934-1-Ig (AGO2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67934-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AGO2/EIF2C2 antibody included
  • FC experiment of HeLa using 67934-1-Ig (same clone as 67934-1-PBS)

    FC experiment of HeLa using 67934-1-Ig (same clone as 67934-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug AGO2 Monoclonal antibody (67934-1-Ig, Clone:1G2H12) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67934-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy AGO2/EIF2C2 Ready-To-Use IHC Kit. AGO2/EIF2C2 primary antibody included.

产品信息

67934-1-PBS targets AGO2 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen AGO2 fusion protein Ag27772 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name eukaryotic translation initiation factor 2C, 2
Synonyms EIF2C2, EC:3.1.26.n2, Argonaute2, Argonaute RISC catalytic component 2, Argonaute 2
Calculated Molecular Weight 97 kDa
Observed Molecular Weight90 kDa
GenBank Accession NumberBC007633
Gene Symbol EIF2C2
Gene ID (NCBI) 27161
RRIDAB_2918686
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UKV8
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EIF2C2, also named as AGO2, belongs to the argonaute family and Ago subfamily. It is required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include EIF2C2/AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). EIF2C2 may inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. It also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. EIF2C2 can also upregulate the translation of specific mRNAs under certain growth conditions.