验证数据展示
产品信息
67934-1-PBS targets AGO2 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, FC (Intra), ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | AGO2 fusion protein Ag27772 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | eukaryotic translation initiation factor 2C, 2 |
别名 | EIF2C2, EC:3.1.26.n2, Argonaute2, Argonaute RISC catalytic component 2, Argonaute 2 |
计算分子量 | 97 kDa |
观测分子量 | 90 kDa |
GenBank蛋白编号 | BC007633 |
基因名称 | EIF2C2 |
Gene ID (NCBI) | 27161 |
RRID | AB_2918686 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q9UKV8 |
储存缓冲液 | PBS only , pH 7.3 |
储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
EIF2C2, also named as AGO2, belongs to the argonaute family and Ago subfamily. It is required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include EIF2C2/AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). EIF2C2 may inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. It also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. EIF2C2 can also upregulate the translation of specific mRNAs under certain growth conditions.