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CoraLite® Plus 488-conjugated AGO2 Monoclonal antibody
AGO2 Monoclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1G2H12
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy AGO2/EIF2C2 Ready-To-Use IHC Kit. AGO2/EIF2C2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67934-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67934 targets AGO2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC, FC (Intra) Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | AGO2 fusion protein Ag27772 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | eukaryotic translation initiation factor 2C, 2 |
| Synonyms | EIF2C2, EC:3.1.26.n2, Argonaute2, Argonaute RISC catalytic component 2, Argonaute 2 |
| Calculated Molecular Weight | 97 kDa |
| Observed Molecular Weight | 90 kDa |
| GenBank Accession Number | BC007633 |
| Gene Symbol | EIF2C2 |
| Gene ID (NCBI) | 27161 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9UKV8 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
EIF2C2, also named as AGO2, belongs to the argonaute family and Ago subfamily. It is required for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include EIF2C2/AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). EIF2C2 may inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. It also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. EIF2C2 can also upregulate the translation of specific mRNAs under certain growth conditions.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 AGO2 antibody CL488-67934 | Download protocol |
| FC protocol for CL Plus 488 AGO2 antibody CL488-67934 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
