验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy AIFM1 Ready-To-Use IHC Kit. AIFM1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 17984-1-AP
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-17984 targets AIF in IF/ICC applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human |
| 免疫原 | AIF fusion protein Ag12400 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | apoptosis-inducing factor, mitochondrion-associated, 1 |
| 别名 | AIF, AIFM1, PDCD8 |
| 计算分子量 | 609 aa, 66 kDa |
| 观测分子量 | 67 kDa, 57 kDa |
| GenBank蛋白编号 | BC111065 |
| 基因名称 | AIF |
| Gene ID (NCBI) | 9131 |
| RRID | AB_3084015 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95831 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Apoptosis-inducing factor (AIF) is one of the mitochondrial proteins to be released into the cytosol during apoptosis, and it is discovered as the first protein that regulates caspase-independent apoptosis(PMID:20494118). AIF is encoded as a 67 kDa protein that contains a mitochondrial localization signal (MLS) in the N-terminus.It is cleaved from the 62 kDa to the 57 kDa form following ischemic injury and translocated from the mitochondria to the nucleus in a calpain-dependent manner(PMID:19332058).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 AIF antibody CL488-17984 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |