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验证数据展示

  • WB analysis using CL488-66902

    WB analysis using CL488-66902

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66902 (AIM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated AIM2 Monoclonal antibody

AIM2 Monoclonal Antibody for WB
Cat No. CL488-66902

Print datasheet

CloneNo. 3E12A8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, rabbit

Applications

WB

Synonyms

PYHIN4, Interferon-inducible protein AIM2, 3E12A8

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inRAW 264.7 cells
Planning an IHC experiment? We recommend our IHCeasy AIM2 Ready-To-Use IHC Kit. AIM2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66902 targets AIM2 in WB applications and shows reactivity with human, mouse, rat, rabbit samples.

Tested Applications WB Application Description
Tested Reactivity human, mouse, rat, rabbit
Immunogen AIM2 fusion protein Ag14607 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name absent in melanoma 2
Synonyms PYHIN4, Interferon-inducible protein AIM2, 3E12A8
Calculated Molecular Weight 343 aa, 39 kDa
Observed Molecular Weight 37-39 and 53 kDa
GenBank Accession NumberBC010940
Gene Symbol AIM2
Gene ID (NCBI) 9447
RRIDAB_3084284
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO14862
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Absent in melanoma 2 (AIM2) is a sensor of cytosolic DNA that is responsible for activation of the inflammasome and host immune responses to DNA viruses and intracellular bacteria [PMID: 22695634]. Upon binding to DNA, it is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Otherwise, it functions as a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity [PMID:16432157,20566831]. 66902-1-Ig antibody detects the 37-39 and 53 kDa protein in SDS-PAGE similar to the paper published. [PMID: 28809949]

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 AIM2 antibody CL488-66902Download protocol
Standard Protocols
Click here to view our Standard Protocols