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验证数据展示

  • WB analysis using CL488-66902

    WB analysis using CL488-66902

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66902 (AIM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated AIM2 Monoclonal antibody

AIM2 Monoclonal Antibody for WB
Cat No. CL488-66902

产品说明书

CloneNo. 3E12A8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, rabbit

应用

WB

别名

PYHIN4, Interferon-inducible protein AIM2, 3E12A8

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inRAW 264.7 cells
Planning an IHC experiment? We recommend our IHCeasy AIM2 Ready-To-Use IHC Kit. AIM2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66902 targets AIM2 in WB applications and shows reactivity with human, mouse, rat, rabbit samples.

经测试应用 WB Application Description
经测试反应性 human, mouse, rat, rabbit
免疫原 AIM2 fusion protein Ag14607 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 absent in melanoma 2
别名 PYHIN4, Interferon-inducible protein AIM2, 3E12A8
计算分子量 343 aa, 39 kDa
观测分子量 37-39 and 53 kDa
GenBank蛋白编号BC010940
基因名称 AIM2
Gene ID (NCBI) 9447
RRIDAB_3084284
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO14862
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Absent in melanoma 2 (AIM2) is a sensor of cytosolic DNA that is responsible for activation of the inflammasome and host immune responses to DNA viruses and intracellular bacteria [PMID: 22695634]. Upon binding to DNA, it is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Otherwise, it functions as a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity [PMID:16432157,20566831]. 66902-1-Ig antibody detects the 37-39 and 53 kDa protein in SDS-PAGE similar to the paper published. [PMID: 28809949]

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 AIM2 antibody CL488-66902Download protocol
Standard Protocols
Click here to view our Standard Protocols