- Featured Product
- KD/KO Validated
CoraLite®594-conjugated AKT Monoclonal antibody
AKT Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
IF/ICC
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
2C5D1
验证数据展示
经过测试的应用
Positive IF/ICC detected in | MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy AKT Ready-To-Use IHC Kit. AKT primary antibody included.
推荐稀释比
Application | Dilution |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of CL594-60203 is AKT Fusion Protein expressed in E. coli.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | AKT fusion protein Ag16695 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | v-akt murine thymoma viral oncogene homolog 1 |
Synonyms | AKT, AKT1, PKB, PKB ALPHA, PRKBA, Protein kinase B, Proto oncogene c Akt, RAC, RAC ALPHA, RAC PK alpha |
Calculated Molecular Weight | 56 kDa |
Observed Molecular Weight | 56-62 kDa |
GenBank Accession Number | BC000479 |
Gene Symbol | AKT1 |
Gene ID (NCBI) | 207 |
RRID | AB_2919902 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P31749 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The serine-threonine protein kinase AKT1 is catalytically inactive in serum-starved primary and immortalized fibroblasts. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. This antibody detects all the members of AKT with/without phospho- modification.
实验方案
Product Specific Protocols | |
---|---|
IF protocol for CL594 AKT antibody CL594-60203 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |