• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

HRP-conjugated APOB Polyclonal antibody

APOB Polyclonal Antibody for WB

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB

Conjugate

HRP

Cat no : HRP-20578

Print datasheet

Synonyms

Apo B 100, Apo B 48, APOB, ApoB100, Apolipoprotein B 100, FLDB

验证数据展示

  • WB analysis of human plasma using HRP-20578

    WB analysis of human plasma using HRP-20578

    human plasma were subjected to SDS PAGE followed by western blot with HRP-20578 (APOB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse liver using HRP-20578

    WB analysis of mouse liver using HRP-20578

    mouse liver tissue were subjected to SDS PAGE followed by western blot with HRP-20578 (APOB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse liver using HRP-20578

    WB analysis of mouse liver using HRP-20578

    mouse liver tissue were subjected to SDS PAGE followed by western blot with HRP-20578 (APOB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse liver tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

HRP-20578 targets APOB in WB applications and shows reactivity with human, mouse samples.

Tested Applications WB Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name apolipoprotein B (including Ag(x) antigen)
Synonyms Apo B 100, Apo B 48, APOB, ApoB100, Apolipoprotein B 100, FLDB
Calculated Molecular Weight 516 kDa
Observed Molecular Weight150-250 kDa, 400-520 kDa
GenBank Accession NumberNM_000384
Gene Symbol APOB
Gene ID (NCBI) 338
RRIDAB_2924163
Conjugate HRP
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP04114
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The apolipoprotein B (APOB) is a plasma protein synthesized primarily in the liver and intestine and play an important role in lipid and cholesterol metabolism. The APOB encodes two different isoproteins through mRNA editing, APOB-48 and APOB-100. APOB-48 and APOB-100 is present in both human liver and intestine. APOB-100 is essential for the assembly of VLDL in the liver. APOB-48 is essential for the assembly of chylomicrons in the intestine. It is well established that APOB-100 levels are associated with coronary heart disease. This antibody recognizes both of APOB-48, APOB-100 and APOB-27.6 (PMID:11839763, PMID:2450346).

实验方案

Product Specific Protocols
WB protocol for HRP APOB antibody HRP-20578Download protocol
Standard Protocols
Click here to view our Standard Protocols