HRP-conjugated APOB Polyclonal antibody
APOB Polyclonal Antibody for WB
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB
Conjugate
HRP
验证数据展示
经过测试的应用
Positive WB detected in | mouse liver tissue |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
HRP-20578 targets APOB in WB applications and shows reactivity with human, mouse samples.
Tested Applications | WB Application Description |
Tested Reactivity | human, mouse |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | apolipoprotein B (including Ag(x) antigen) |
Synonyms | Apo B 100, Apo B 48, APOB, ApoB100, Apolipoprotein B 100, FLDB |
Calculated Molecular Weight | 516 kDa |
Observed Molecular Weight | 150-250 kDa, 400-520 kDa |
GenBank Accession Number | NM_000384 |
Gene Symbol | APOB |
Gene ID (NCBI) | 338 |
Conjugate | HRP |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P04114 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The apolipoprotein B (APOB) is a plasma protein synthesized primarily in the liver and intestine and play an important role in lipid and cholesterol metabolism. The APOB encodes two different isoproteins through mRNA editing, APOB-48 and APOB-100. APOB-48 and APOB-100 is present in both human liver and intestine. APOB-100 is essential for the assembly of VLDL in the liver. APOB-48 is essential for the assembly of chylomicrons in the intestine. It is well established that APOB-100 levels are associated with coronary heart disease. This antibody recognizes both of APOB-48, APOB-100 and APOB-27.6 (PMID:11839763, PMID:2450346).
实验方案
Product Specific Protocols | |
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WB protocol for HRP APOB antibody HRP-20578 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |