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验证数据展示

  • WB analysis of human plasma using HRP-20578

    WB analysis of human plasma using HRP-20578

    human plasma were subjected to SDS PAGE followed by western blot with HRP-20578 (APOB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse liver using HRP-20578

    WB analysis of mouse liver using HRP-20578

    mouse liver tissue were subjected to SDS PAGE followed by western blot with HRP-20578 (APOB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse liver using HRP-20578

    WB analysis of mouse liver using HRP-20578

    mouse liver tissue were subjected to SDS PAGE followed by western blot with HRP-20578 (APOB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

HRP-conjugated APOB Polyclonal antibody

APOB Polyclonal Antibody for WB
Cat No. HRP-20578

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB

别名

Apo B 100, Apo B 48, APOB, ApoB100, Apolipoprotein B 100, FLDB

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  HRP
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse liver tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

HRP-20578 targets APOB in WB applications and shows reactivity with human, mouse samples.

经测试应用 WB Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 apolipoprotein B (including Ag(x) antigen)
别名 Apo B 100, Apo B 48, APOB, ApoB100, Apolipoprotein B 100, FLDB
计算分子量 516 kDa
观测分子量150-250 kDa, 400-520 kDa
GenBank蛋白编号NM_000384
基因名称 APOB
Gene ID (NCBI) 338
RRIDAB_2924163
偶联类型 HRP
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP04114
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The apolipoprotein B (APOB) is a plasma protein synthesized primarily in the liver and intestine and play an important role in lipid and cholesterol metabolism. The APOB encodes two different isoproteins through mRNA editing, APOB-48 and APOB-100. APOB-48 and APOB-100 is present in both human liver and intestine. APOB-100 is essential for the assembly of VLDL in the liver. APOB-48 is essential for the assembly of chylomicrons in the intestine. It is well established that APOB-100 levels are associated with coronary heart disease. This antibody recognizes both of APOB-48, APOB-100 and APOB-27.6 (PMID:11839763, PMID:2450346).

实验方案

Product Specific Protocols
WB protocol for HRP APOB antibody HRP-20578Download protocol
Standard Protocols
Click here to view our Standard Protocols