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验证数据展示

  • IF Staining of human liver cancer using CL488-16001

    IF Staining of human liver cancer using CL488-16001

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite® Plus 488 ARG1 antibody (CL488-16001) at dilution of 1:400.

  • IF Staining of HepG2 using CL488-16001

    IF Staining of HepG2 using CL488-16001

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 Arginase-1 antibody (CL488-16001) at dilution of 1:200.

CoraLite® Plus 488-conjugated Arginase-1 Polyclonal antibody

Arginase-1 Polyclonal Antibody for IF/ICC, IF-P
Cat No. CL488-16001

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, IF-P

别名

ARG1, Arginase 1, liver Arginase, arginase,liver, arginase, liver

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman liver cancer tissue
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ARG1 Ready-To-Use IHC Kit. ARG1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 16001-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-16001 targets Arginase-1 in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, IF-P Application Description
经测试反应性 human, mouse, rat
免疫原 Arginase-1 fusion protein Ag8595 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 arginase, liver
别名 ARG1, Arginase 1, liver Arginase, arginase,liver, arginase, liver
计算分子量236aa,25 kDa; 322aa,35 kDa
观测分子量 35-36 kDa
GenBank蛋白编号BC005321
基因名称 Arginase-1
Gene ID (NCBI) 383
RRIDAB_2919127
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP05089
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Arginase-1 (Liver arginase) belongs to the arginase family. ARG1 is a novel immunohistochemical marker of hepatocellular differentiation in fine needle aspiration cytology and a marker of hepatocytes and hepatocellular neoplasms. ARG1 is closely associated with alternative macrophage activation(PMID:12098359) and ARG1 has been shown to protect motor neurons from trophic factor deprivation and allow sensory neurons to overcome neurite outgrowth inhibition by myelin proteins(PMID: 20071539). It can exist as a homotrimer(PMID:16141327) and it has 3 isoforms produced by alternative splicing. Defects in ARG1 are the cause of argininemia (ARGIN). Deletion or TNF-mediated restriction of ARG1 unleashes the production of NO by NOS2, which is critical for pathogen control.(PMID:27117406). ARG1 has 3 isoforms with the molecular mass of 25, 35 and 36 kDa.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Arginase-1 antibody CL488-16001Download protocol
Standard Protocols
Click here to view our Standard Protocols