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验证数据展示

  • WB analysis using 31206-1-AP

    WB analysis using 31206-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31206-1-AP (ASCL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

ASCL1 Polyclonal antibody

ASCL1 Polyclonal Antibody for WB, ELISA
Cat No. 31206-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, ELISA

别名

Achaete scute homolog 1, ASCL1, ASH 1, ASH1, bHLHa46, HASH1, MASH1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, NCI-H1299 cells, U-251 cells, PC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31206-1-AP targets ASCL1 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 ASCL1 fusion protein Ag35185 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 achaete-scute complex homolog 1 (Drosophila)
别名 Achaete scute homolog 1, ASCL1, ASH 1, ASH1, bHLHa46, HASH1, MASH1
计算分子量 25 kDa
观测分子量25 kDa
GenBank蛋白编号BC003134
基因名称 ASCL1
Gene ID (NCBI) 429
RRIDAB_3669901
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP50553
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ASCL1 (achaete-scute family bHLH transcription factor 1), also known as ASH1. It is expected to be located in nucleus. Transcription factor that plays a key role in neuronal differentiation: acts as a pioneer transcription factor, accessing closed chromatin to allow other factors to bind and activate neural pathways. Plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation. Involved in the regulation of neuroendocrine cell development in the glandular stomach. The calculated molecular weight of ASCL1 is 25 kDa.

实验方案

Product Specific Protocols
WB protocol for ASCL1 antibody 31206-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols