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ASCL1 Polyclonal antibody

ASCL1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31206-1-AP

Print datasheet

Synonyms

Achaete scute homolog 1, ASCL1, ASH 1, ASH1, bHLHa46, HASH1, MASH1

验证数据展示

  • WB analysis using 31206-1-AP

    WB analysis using 31206-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31206-1-AP (ASCL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inK-562 cells, NCI-H1299 cells, U-251 cells, PC-3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31206-1-AP targets ASCL1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen ASCL1 fusion protein Ag35185 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name achaete-scute complex homolog 1 (Drosophila)
Synonyms Achaete scute homolog 1, ASCL1, ASH 1, ASH1, bHLHa46, HASH1, MASH1
Calculated Molecular Weight 25 kDa
Observed Molecular Weight25 kDa
GenBank Accession NumberBC003134
Gene Symbol ASCL1
Gene ID (NCBI) 429
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP50553
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ASCL1 (achaete-scute family bHLH transcription factor 1), also known as ASH1. It is expected to be located in nucleus. Transcription factor that plays a key role in neuronal differentiation: acts as a pioneer transcription factor, accessing closed chromatin to allow other factors to bind and activate neural pathways. Plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation. Involved in the regulation of neuroendocrine cell development in the glandular stomach. The calculated molecular weight of ASCL1 is 25 kDa.

实验方案

Product Specific Protocols
WB protocol for ASCL1 antibody 31206-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols