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验证数据展示

  • WB analysis of mouse brain using 66412-1-Ig (same clone as 66412-1-PBS)

    WB analysis of mouse brain using 66412-1-Ig (same clone as 66412-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66412-1-Ig (a-Synuclein Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66412-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 66412-1-Ig (same clone as 66412-1-PBS)

    IHC staining of mouse cerebellum using 66412-1-Ig (same clone as 66412-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66412-1-Ig (alpha-Synuclein antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66412-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Alpha Synuclein antibody included
  • IHC staining of mouse cerebellum using 66412-1-Ig (same clone as 66412-1-PBS)

    IHC staining of mouse cerebellum using 66412-1-Ig (same clone as 66412-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 66412-1-Ig (alpha-Synuclein antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66412-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Alpha Synuclein antibody included
  • IF Staining of mouse cerebellum using 66412-1-Ig (same clone as 66412-1-PBS)

    IF Staining of mouse cerebellum using 66412-1-Ig (same clone as 66412-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using Alpha Synuclein antibody (66412-1-Ig, Clone: 1B10E9 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66412-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse cerebellum using 66412-1-Ig (same clone as 66412-1-PBS)

    IF Staining of mouse cerebellum using 66412-1-Ig (same clone as 66412-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using Alpha Synuclein antibody (66412-1-Ig, Clone: 1B10E9 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66412-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50043-1

    Sandwich ELISA standard curve of MP50043-1

    Sandwich ELISA standard curve of MP50043-1, a-Synuclein Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60285-1-PBS. Detection antibody: 66412-1-PBS. Standard: Ag1285. Range: 0.313-20 ng/mL.

  • IHC staining of human brain using 66412-1-Ig (same clone as 66412-1-PBS)

    IHC staining of human brain using 66412-1-Ig (same clone as 66412-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66412-1-Ig (a-Synuclein antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66412-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Alpha Synuclein antibody included

Alpha Synuclein Monoclonal antibody, PBS Only (Detector)

Alpha Synuclein Monoclonal Antibody for WB, IHC, IF-P, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 66412-1-PBS

产品说明书

CloneNo. 1B10E9

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Sandwich ELISA, Indirect ELISA, Sample test

别名

a-synuclein, SNCA, Non-A4 component of amyloid precursor, Non-A beta component of AD amyloid, NACP

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy Alpha Synuclein Ready-To-Use IHC Kit. Alpha Synuclein primary antibody included.

产品信息

66412-1-PBS targets Alpha Synuclein as part of a matched antibody pair:

MP50043-1: 60285-1-PBS capture and 66412-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF-P, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse, rat
免疫原 Alpha Synuclein fusion protein Ag1285 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 synuclein, alpha (non A4 component of amyloid precursor)
别名 a-synuclein, SNCA, Non-A4 component of amyloid precursor, Non-A beta component of AD amyloid, NACP
计算分子量 14 kDa
观测分子量 18-19 kDa
GenBank蛋白编号BC013293
基因名称 Alpha Synuclein
Gene ID (NCBI) 6622
RRIDAB_2881784
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP37840
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Alpha Synuclein (α-syn) is a 14-19 kDa phosphoprotein that is primarily localize to the presynaptic terminals of mature neurons, where it is involved in synaptic function and plasticity. α-syn has drawn intense interest ever since the late 1990s, when the first α-synuclein missense mutation was identified as a cause of familial Parkinson's disease (PD). Aggregated and hyper-phosphorylated forms of α-syn protein are the pathological hallmark of Lewy body disease, which includes Parkinson's disease (PD), diffuse Lewy body disease (DLBD), and Lewy body variant of Alzheimer's disease (LBV). This antibody can recognize all the isoforms of α-syn.