Alpha Synuclein Monoclonal antibody
Alpha Synuclein Monoclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, rat, mouse
Applications
WB, IHC, IF-P, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
1B10E9
验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, rat brain tissue |
Positive IHC detected in | mouse cerebellum tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse cerebellum tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IHC | See 2 publications below |
IF | See 1 publications below |
产品信息
66412-1-Ig targets Alpha Synuclein in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat, mouse samples.
Tested Applications | WB, IHC, IF-P, ELISA Application Description |
Cited Applications | IHC, IF |
Tested Reactivity | human, rat, mouse |
Cited Reactivity | human, rat |
Immunogen | Alpha Synuclein fusion protein Ag1285 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | synuclein, alpha (non A4 component of amyloid precursor) |
Synonyms | a-synuclein, SNCA, Non-A4 component of amyloid precursor, Non-A beta component of AD amyloid, NACP |
Calculated Molecular Weight | 14 kDa |
Observed Molecular Weight | 18-19 kDa |
GenBank Accession Number | BC013293 |
Gene Symbol | Alpha Synuclein |
Gene ID (NCBI) | 6622 |
RRID | AB_2881784 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P37840 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Alpha Synuclein antibody 66412-1-Ig | Download protocol |
IHC protocol for Alpha Synuclein antibody 66412-1-Ig | Download protocol |
IF protocol for Alpha Synuclein antibody 66412-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Appl Microbiol Biotechnol The alteration of intestinal mucosal α-synuclein expression and mucosal microbiota in Parkinson's disease | ||
Biomed Pharmacother Nardosinone regulates the slc38a2 gene to alleviate Parkinson's symptoms in rats through the GABAergic synaptic and cAMP pathways. | ||
J Neuropathol Exp Neurol Regular Aerobic Exercise-Alleviated Dysregulation of CAMKIIα Carbonylation to Mitigate Parkinsonism via Homeostasis of Apoptosis With Autophagy. |