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  • Featured Product
  • KD/KO Validated

Annexin A1 Monoclonal antibody, PBS Only

Annexin A1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E1B7

Cat no : 66344-1-PBS

Print datasheet

Synonyms

ANXA1, ANX1, Annexin-1, Annexin I, Annexin Ac2-26

验证数据展示

  • WB analysis using 66344-1-Ig (same clone as 66344-1-PBS)

    WB analysis using 66344-1-Ig (same clone as 66344-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66344-1-Ig (Annexin A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66344-1-PBS in a different storage buffer formulation.

  • WB analysis using 66344-1-Ig (same clone as 66344-1-PBS)

    WB analysis using 66344-1-Ig (same clone as 66344-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66344-1-Ig (Annexin A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66344-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66344-1-Ig (same clone as 66344-1-PBS)

    WB analysis of HeLa using 66344-1-Ig (same clone as 66344-1-PBS)

    WB result of Annexin A1 antibody (66344-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Annexin A1 transfected HeLa cells. This data was developed using the same antibody clone with 66344-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach using 66344-1-Ig (same clone as 66344-1-PBS)

    IHC staining of human stomach using 66344-1-Ig (same clone as 66344-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66344-1-Ig (Annexin A1 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66344-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ANXA1 antibody included
  • IHC staining of human stomach using 66344-1-Ig (same clone as 66344-1-PBS)

    IHC staining of human stomach using 66344-1-Ig (same clone as 66344-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66344-1-Ig (Annexin A1 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66344-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ANXA1 antibody included
  • IHC staining of mouse liver using 66344-1-Ig (same clone as 66344-1-PBS)

    IHC staining of mouse liver using 66344-1-Ig (same clone as 66344-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66344-1-Ig (Annexin A1 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66344-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ANXA1 antibody included
  • IHC staining of mouse liver using 66344-1-Ig (same clone as 66344-1-PBS)

    IHC staining of mouse liver using 66344-1-Ig (same clone as 66344-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66344-1-Ig (Annexin A1 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66344-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ANXA1 antibody included
  • FC experiment of K-562 using 66344-1-Ig (same clone as 66344-1-PBS)

    FC experiment of K-562 using 66344-1-Ig (same clone as 66344-1-PBS)

    1X10^6 K-562 cells were intracellularly stained with 0.8 ug Anti-Human Annexin A1 (66344-1-Ig, Clone:1E1B7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66344-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 66344-1-Ig (same clone as 66344-1-PBS)

    IF Staining of A549 using 66344-1-Ig (same clone as 66344-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Annexin A1 antibody (66344-1-Ig, Clone: 1E1B7 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66344-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy ANXA1 Ready-To-Use IHC Kit. ANXA1 primary antibody included.

产品信息

66344-1-PBS targets Annexin A1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Annexin A1 fusion protein Ag17273 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name annexin A1
Synonyms ANXA1, ANX1, Annexin-1, Annexin I, Annexin Ac2-26
Calculated Molecular Weight 346 aa, 39 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC001275
Gene Symbol Annexin A1
Gene ID (NCBI) 301
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04083
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ANXA1, also named as ANX1, LPC1 and p35, belongs to the annexin family. It is a member of the annexin family of Ca2+-binding and phospholipidbinding proteins, and it is particularly abundant in various populations of peripheral blood leukocytes. ANXA1 regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. ANXA1 is a pleiotrophic protein produced by many cell types including peripheral blood leucocytes.