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验证数据展示

  • IF Staining of A549 using CL594-66344

    IF Staining of A549 using CL594-66344

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite®594 Annexin A1 antibody (CL594-66344, Clone: 1E1B7 ) at dilution of 1:200.

  • FC experiment of K-562 using CL594-66344

    FC experiment of K-562 using CL594-66344

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Annexin A1 (CL594-66344, Clone:1E1B7) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated Annexin A1 Monoclonal antibody

Annexin A1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-66344

产品说明书

CloneNo. 1E1B7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

别名

ANXA1, ANX1, Annexin-1, Annexin I, Annexin Ac2-26

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy ANXA1 Ready-To-Use IHC Kit. ANXA1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66344-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66344 targets Annexin A1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 Annexin A1 fusion protein Ag17273 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 annexin A1
别名 ANXA1, ANX1, Annexin-1, Annexin I, Annexin Ac2-26
计算分子量 346 aa, 39 kDa
观测分子量 35 kDa
GenBank蛋白编号BC001275
基因名称 Annexin A1
Gene ID (NCBI) 301
RRIDAB_2883563
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP04083
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ANXA1, also named as ANX1, LPC1 and p35, belongs to the annexin family. It is a member of the annexin family of Ca2+-binding and phospholipidbinding proteins, and it is particularly abundant in various populations of peripheral blood leukocytes. ANXA1 regulates phospholipase A2 activity. It seems to bind from two to four calcium ions with high affinity. ANXA1 is a pleiotrophic protein produced by many cell types including peripheral blood leucocytes.

实验方案

Product Specific Protocols
IF protocol for CL594 Annexin A1 antibody CL594-66344Download protocol
Standard Protocols
Click here to view our Standard Protocols