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验证数据展示

  • WB analysis using 60051-1-Ig (same clone as 60051-1-PBS)

    WB analysis using 60051-1-Ig (same clone as 60051-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60051-1-Ig (Annexin A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.

  • WB analysis of THP-1 using 60051-1-Ig (same clone as 60051-1-PBS)

    WB analysis of THP-1 using 60051-1-Ig (same clone as 60051-1-PBS)

    WB result of Annexin A2 antibody (60051-1-Ig; 1:10000; room temperature for 1.5 hours) with wild-type and sg-Annexin A2 transfected THP-1 cells. This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 60051-1-Ig (same clone as 60051-1-PBS)

    WB analysis of HEK-293 using 60051-1-Ig (same clone as 60051-1-PBS)

    WB result of Annexin A2 antibody (60051-1-Ig, 1:20,000) with si-Control and si-Annexin A2 transfected HEK293 cells. This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 60051-1-Ig (same clone as 60051-1-PBS)

    IHC staining of human breast cancer using 60051-1-Ig (same clone as 60051-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60051-1-Ig (Annexin A2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ANXA2 antibody included
  • IHC staining of human breast cancer using 60051-1-Ig (same clone as 60051-1-PBS)

    IHC staining of human breast cancer using 60051-1-Ig (same clone as 60051-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60051-1-Ig (Annexin A2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ANXA2 antibody included
  • IF Staining of T-47D using 60051-1-Ig (same clone as 60051-1-PBS)

    IF Staining of T-47D using 60051-1-Ig (same clone as 60051-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed T-47D cells using 60051-1-Ig (Annexin A2 antibody), at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 60051-1-Ig (same clone as 60051-1-PBS)

    IP experiment of HeLa using 60051-1-Ig (same clone as 60051-1-PBS)

    IP result of anti-Annexin A2 (IP:60051-1-Ig, 4ug; Detection:60051-1-Ig 1:1500) with HeLa cells lysate 2400ug. This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 60051-1-Ig (same clone as 60051-1-PBS)

    IF Staining of MCF-7 using 60051-1-Ig (same clone as 60051-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using Annexin A2 antibody (60051-1-Ig, Clone: 3H1B11 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60051-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Annexin A2 Monoclonal antibody, PBS Only

Annexin A2 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 60051-1-PBS

产品说明书

CloneNo. 3H1B11

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

ANXA2, 3H1B11, Annexin 2, Annexin II, Annexin-2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ANXA2 Ready-To-Use IHC Kit. ANXA2 primary antibody included.

产品信息

60051-1-PBS targets Annexin A2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1777

Product name: Recombinant human Annexin A2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-339 aa of BC016774

Sequence: MSTVHEILCKLSLEGDHSTPPSAYGSVKAYTNFDAERDALNIETAIKTKGVDEVTIVNILTNRSNAQRQDIAFAYQRRTKKELASALKSALSGHLETVILGLLKTPAQYDASELKASMKGLGTDEDSLIEIICSRTNQELQEINRVYKEMYKTDLEKDIISDTSGDFRKLMVALAKGRRAEDGSVIDYELIDQDARDLYDAGVKRKGTDVPKWISIMTERSVPHLQKVFDRYKSYSPYDMLESIRKEVKGDLENAFLNLVQCIQNKPLYFADRLYDSMKGKGTRDKVLIRIMVSRSEVDMLKIRSEFKRKYGKSLYYYIQQDTKGDYQKALLYLCGGDD

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 annexin A2
别名 ANXA2, 3H1B11, Annexin 2, Annexin II, Annexin-2
计算分子量 339 aa, 39 kDa
观测分子量 35-38 kDa
GenBank蛋白编号BC016774
基因名称 Annexin A2
Gene ID (NCBI) 302
ENSEMBL Gene IDENSG00000182718
RRIDAB_2057309
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP07355
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.