验证数据展示
经过测试的应用
Positive WB detected in | K-562 cells, HEK-293 cells, THP-1 cells, A431 cells, A549 cells, HeLa cells, HepG2 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human breast cancer tissue, human lung cancer tissue, human skin cancer tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | T-47D cells, HeLa cells, MCF-7 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:5000-1:20000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
60051-1-Ig targets Annexin A2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
文献引用应用 | WB, IF, IP, CoIP |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse |
免疫原 | Annexin A2 fusion protein Ag1777 种属同源性预测 |
宿主/亚型 | Mouse / IgG2b |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | annexin A2 |
别名 | ANXA2, ANX2L4, ANX2, Annexin-2, Annexin II |
计算分子量 | 339 aa, 39 kDa |
观测分子量 | 35-38 kDa |
GenBank蛋白编号 | BC016774 |
基因名称 | Annexin A2 |
Gene ID (NCBI) | 302 |
ENSEMBL Gene ID | ENSG00000182718 |
RRID | AB_2057309 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P07355 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Annexin A2 antibody 60051-1-Ig | Download protocol |
IHC protocol for Annexin A2 antibody 60051-1-Ig | Download protocol |
IF protocol for Annexin A2 antibody 60051-1-Ig | Download protocol |
IP protocol for Annexin A2 antibody 60051-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Streptococcus anginosus promotes gastric inflammation, atrophy, and tumorigenesis in mice | ||
EBioMedicine Compounds targeting YadC of uropathogenic Escherichia coli and its host receptor annexin A2 decrease bacterial colonization in bladder. | ||
Infect Immun The Tick Protein Sialostatin L2 Binds to Annexin A2 and Inhibits NLRC4-Mediated Inflammasome Activation. | ||
Cell Mol Life Sci Upregulation of HDAC9 in hippocampal neurons mediates depression-like behaviours by inhibiting ANXA2 degradation | ||
iScience Opportunistic pathogen Porphyromonas gingivalis targets the LC3B-ceramide complex and mediates lethal mitophagy resistance in oral tumors
| ||
Cell Signal The novel DNA methylation marker FIBIN suppresses non-small cell lung cancer metastasis by negatively regulating ANXA2 |