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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Annexin A2 Monoclonal antibody

Annexin A2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

IF/ICC and More (1)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3H1B11

Cat no : CL488-60051

Print datasheet

Synonyms

ANXA2, ANX2L4, ANX2, Annexin-2, Annexin II

验证数据展示

  • IF Staining of MCF-7 using CL488-60051

    IF Staining of MCF-7 using CL488-60051

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 Annexin A2 antibody (CL488-60051, Clone: 3H1B11 ) at dilution of 1:100.

经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy ANXA2 Ready-To-Use IHC Kit. ANXA2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60051-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

CL488-60051 targets Annexin A2 in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen Annexin A2 fusion protein Ag1777 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name annexin A2
Synonyms ANXA2, ANX2L4, ANX2, Annexin-2, Annexin II
Calculated Molecular Weight 339 aa, 39 kDa
Observed Molecular Weight 35-38 kDa
GenBank Accession NumberBC016774
Gene Symbol Annexin A2
Gene ID (NCBI) 302
ENSEMBL Gene IDENSG00000182718
RRIDAB_2883091
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07355
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Annexin A2 antibody CL488-60051Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Cancer Sci

GNAQ T96S mutation abrogates the ability of wild-type GNAQ to induce apoptosis by phosphorylating annexin A2 in natural killer/T cell lymphoma.

Authors - Wugan Zhao
View Article