验证数据展示
经过测试的应用
| Positive IF/ICC detected in | MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy ANXA2 Ready-To-Use IHC Kit. ANXA2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60051-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL488-60051 targets Annexin A2 in IF/ICC applications and shows reactivity with human, mouse samples.
| Tested Applications | IF/ICC Application Description |
| Cited Applications | IF |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Immunogen | Annexin A2 fusion protein Ag1777 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | annexin A2 |
| Synonyms | ANXA2, ANX2L4, ANX2, Annexin-2, Annexin II |
| Calculated Molecular Weight | 339 aa, 39 kDa |
| Observed Molecular Weight | 35-38 kDa |
| GenBank Accession Number | BC016774 |
| Gene Symbol | Annexin A2 |
| Gene ID (NCBI) | 302 |
| ENSEMBL Gene ID | ENSG00000182718 |
| RRID | AB_2883091 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P07355 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Annexin A2 (ANXA2), also named Annexin-2 or Lipocortin II, is a Ca2+ binding protein that is up-regulated in virally transformed cell lines and human tumors. This calcium-regulated membrane-binding protein, whose affinity for calcium is greatly enhanced by anionic phospholipids, may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis. Heat-stress response may also involve Annexin-2.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Annexin A2 antibody CL488-60051 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |