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验证数据展示

  • WB analysis using 66245-1-Ig (same clone as 66245-1-PBS)

    WB analysis using 66245-1-Ig (same clone as 66245-1-PBS)

    Various cells were subjected to SDS PAGE followed by western blot with 66245-1-Ig (Annexin V antibody) at dilution of 1:13000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66245-1-Ig (same clone as 66245-1-PBS)

    WB analysis of HeLa using 66245-1-Ig (same clone as 66245-1-PBS)

    WB result of Annexin V antibody (66245-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Annexin V transfected HeLa cells. This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66245-1-Ig (same clone as 66245-1-PBS)

    IF Staining of HeLa using 66245-1-Ig (same clone as 66245-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Annexin V antibody (66245-1-Ig, Clone: 1E6A8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.

  • WB analysis using 66245-1-Ig (same clone as 66245-1-PBS)

    WB analysis using 66245-1-Ig (same clone as 66245-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66245-1-Ig (Annexin V antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.

  • IHC staining of human malignant melanoma using 66245-1-Ig (same clone as 66245-1-PBS)

    IHC staining of human malignant melanoma using 66245-1-Ig (same clone as 66245-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66245-1-Ig (Annexin V antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.

  • IHC staining of human malignant melanoma using 66245-1-Ig (same clone as 66245-1-PBS)

    IHC staining of human malignant melanoma using 66245-1-Ig (same clone as 66245-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66245-1-Ig (Annexin V antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.

  • FC experiment of NIH/3T3 using 66245-1-Ig (same clone as 66245-1-PBS)

    FC experiment of NIH/3T3 using 66245-1-Ig (same clone as 66245-1-PBS)

    1x10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug Annexin V Monoclonal antibody (66245-1-Ig, Clone:1E6A8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 66245-1-Ig (same clone as 66245-1-PBS)

    FC experiment of HeLa using 66245-1-Ig (same clone as 66245-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Annexin V Monoclonal antibody (66245-1-Ig, Clone:1E6A8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Annexin V Monoclonal antibody, PBS Only

Annexin V Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 66245-1-PBS

产品说明书

CloneNo. 1E6A8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

Annexin A5, ANXA5, Anchorin CII, Annexin 5, Annexin-5

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66245-1-PBS targets Annexin V in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 Annexin V fusion protein Ag1538 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 annexin A5
别名 Annexin A5, ANXA5, Anchorin CII, Annexin 5, Annexin-5
计算分子量 36 kDa
观测分子量 36 kDa
GenBank蛋白编号BC001429
基因名称 Annexin V
Gene ID (NCBI) 308
RRIDAB_2881634
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP08758
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Annexin A5 (ANXA5), is a member of the structurally related family of annexin proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII.