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Species Reactivity
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验证数据展示

  • WB analysis using 66245-1-Ig (same clone as 66245-1-PBS)

    WB analysis using 66245-1-Ig (same clone as 66245-1-PBS)

    Various cells were subjected to SDS PAGE followed by western blot with 66245-1-Ig (Annexin V antibody) at dilution of 1:13000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66245-1-Ig (same clone as 66245-1-PBS)

    WB analysis of HeLa using 66245-1-Ig (same clone as 66245-1-PBS)

    WB result of Annexin V antibody (66245-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Annexin V transfected HeLa cells. This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66245-1-Ig (same clone as 66245-1-PBS)

    IF Staining of HeLa using 66245-1-Ig (same clone as 66245-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Annexin V antibody (66245-1-Ig, Clone: 1E6A8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.

  • WB analysis using 66245-1-Ig (same clone as 66245-1-PBS)

    WB analysis using 66245-1-Ig (same clone as 66245-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66245-1-Ig (Annexin V antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.

  • IHC staining of human malignant melanoma using 66245-1-Ig (same clone as 66245-1-PBS)

    IHC staining of human malignant melanoma using 66245-1-Ig (same clone as 66245-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66245-1-Ig (Annexin V antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.

  • IHC staining of human malignant melanoma using 66245-1-Ig (same clone as 66245-1-PBS)

    IHC staining of human malignant melanoma using 66245-1-Ig (same clone as 66245-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 66245-1-Ig (Annexin V antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.

  • FC experiment of NIH/3T3 using 66245-1-Ig (same clone as 66245-1-PBS)

    FC experiment of NIH/3T3 using 66245-1-Ig (same clone as 66245-1-PBS)

    1x10^6 NIH/3T3 cells were intracellularly stained with 0.25 ug Annexin V Monoclonal antibody (66245-1-Ig, Clone:1E6A8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 66245-1-Ig (same clone as 66245-1-PBS)

    FC experiment of HeLa using 66245-1-Ig (same clone as 66245-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Annexin V Monoclonal antibody (66245-1-Ig, Clone:1E6A8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66245-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

Annexin V Monoclonal antibody, PBS Only

Annexin V Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 66245-1-PBS

Print datasheet

CloneNo. 1E6A8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

Annexin A5, ANXA5, ANX5, Annexin-5, Annexin 5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

66245-1-PBS targets Annexin V in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen Annexin V fusion protein Ag1538 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name annexin A5
Synonyms Annexin A5, ANXA5, ANX5, Annexin-5, Annexin 5
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC001429
Gene Symbol Annexin V
Gene ID (NCBI) 308
RRIDAB_2881634
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP08758
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Annexin A5 (ANXA5), is a member of the structurally related family of annexin proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII.