- Featured Product
- KD/KO Validated
CoraLite® Plus 647-conjugated Annexin V Polyclonal antibody
Annexin V Polyclonal Antibody for IF/ICC, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat, pig
Applications
IF/ICC, FC (Intra)
Conjugate
CoraLite® Plus 647 Fluorescent Dye
验证数据展示
经过测试的应用
Positive IF/ICC detected in | HeLa cells |
Positive FC (Intra) detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 11060-1-AP
推荐稀释比
Application | Dilution |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL647-11060 targets Annexin V in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.
Tested Applications | IF/ICC, FC (Intra) Application Description |
Tested Reactivity | human, mouse, rat, pig |
Immunogen | Annexin V fusion protein Ag1538 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | annexin A5 |
Synonyms | Annexin A5, ANXA5, ANX5, Annexin-5, Annexin 5 |
Calculated Molecular Weight | 36 kDa |
Observed Molecular Weight | 36 kDa |
GenBank Accession Number | BC018671 |
Gene Symbol | Annexin V |
Gene ID (NCBI) | 308 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P08758 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Annexin A5 (ANXA5), is a member of the structurally related family of annexin proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII.
实验方案
Product Specific Protocols | |
---|---|
IF protocol for CL Plus 647 Annexin V antibody CL647-11060 | Download protocol |
FC protocol for CL Plus 647 Annexin V antibody CL647-11060 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |