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验证数据展示

  • IF Staining of rat liver using CL532-66129

    IF Staining of rat liver using CL532-66129

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat liver tissue using CoraLite®532 Arginase-1 antibody (CL532-66129, Clone: 5D6D12 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  • KD/KO验证

CoraLite®532-conjugated Arginase-1 Monoclonal antibody

Arginase-1 Monoclonal Antibody for IF-P
Cat No. CL532-66129

产品说明书

CloneNo. 5D6D12

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

IF-P

别名

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®532
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inrat liver tissue
Planning an IHC experiment? We recommend our IHCeasy ARG1 Ready-To-Use IHC Kit. ARG1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66129-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL532-66129 targets Arginase-1 in IF-P applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 IF-P Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 Arginase-1 fusion protein Ag8810 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 arginase, liver
别名
计算分子量236aa,25 kDa; 322aa,35 kDa
观测分子量 36 kDa
GenBank蛋白编号BC005321
基因名称 Arginase-1
Gene ID (NCBI) 383
偶联类型 CoraLite®532 Fluorescent Dye
最大激发/发射波长537 nm / 560 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP05089
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Arginase-1 (Liver arginase) belongs to the arginase family. ARG1 is a novel immunohistochemical marker of hepatocellular differentiation in fine needle aspiration cytology and a marker of hepatocytes and hepatocellular neoplasms. ARG1 is closely associated with alternative macrophage activation and ARG1 has been shown to protectmotor neurons from trophic factor deprivation and allow sensory neurons to overcome neurite outgrowth inhibition by myelin proteins (PMID: 20071539, PMID:12098359). It can exsit as a homotrimer and it has 3 isoforms produced by alternative splicing (PMID:16141327). Defects in ARG1 are the cause of argininemia (ARGIN). Deletion or TNF-mediated restriction of ARG1 unleashes the production of NO by NOS2, which is critical for pathogen control (PMID:27117406). Before stroke, ARG1 mainly expressed in neurons in a normal brain (PMID: 23311438). The expression of ARG1 increases in microglia/macrophages and astrocytes early after CNS injuries. ARG1 has been regarded as a marker for beneficial microglia/macrophages and possesses antiinflammatory and tissue repair properties under various pathological conditions (PMID: 26538310, PMID: 31619589).

实验方案

Product Specific Protocols
IF protocol for CL532 Arginase-1 antibody CL532-66129Download protocol
Standard Protocols
Click here to view our Standard Protocols