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验证数据展示

  • WB analysis using 60096-1-Ig (same clone as 60096-1-PBS)

    WB analysis using 60096-1-Ig (same clone as 60096-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60096-1-Ig (B23/NPM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 60096-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 60096-1-Ig (same clone as 60096-1-PBS)

    WB analysis of HepG2 using 60096-1-Ig (same clone as 60096-1-PBS)

    WB result of B23/NPM1 antibody (60096-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-B23/NPM1 transfected HepG2 cells. This data was developed using the same antibody clone with 60096-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 60096-1-Ig (same clone as 60096-1-PBS)

    IHC staining of human breast cancer using 60096-1-Ig (same clone as 60096-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60096-1-Ig (B23 Antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60096-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NPM1 antibody included
  • IHC staining of human breast cancer using 60096-1-Ig (same clone as 60096-1-PBS)

    IHC staining of human breast cancer using 60096-1-Ig (same clone as 60096-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60096-1-Ig (B23 Antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60096-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with NPM1 antibody included
  • WB analysis using 60096-1-Ig (same clone as 60096-1-PBS)

    WB analysis using 60096-1-Ig (same clone as 60096-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60096-1-Ig (B23/NPM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60096-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 60096-1-Ig (same clone as 60096-1-PBS)

    IF Staining of HepG2 using 60096-1-Ig (same clone as 60096-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using B23/NPM1 antibody (60096-1-Ig, Clone: 4F12A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60096-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

B23/NPM1 Monoclonal antibody, PBS Only

B23/NPM1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 60096-1-PBS

Print datasheet

CloneNo. 4F12A3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

B23, NPM1, Numatrin, Nucleophosmin, NPM

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy NPM1 Ready-To-Use IHC Kit. NPM1 primary antibody included.

产品信息

60096-1-PBS targets B23/NPM1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen B23/NPM1 fusion protein Ag7415 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name nucleophosmin (nucleolar phosphoprotein B23, numatrin)
Synonyms B23, NPM1, Numatrin, Nucleophosmin, NPM
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 35-38 kDa
GenBank Accession NumberBC002398
Gene Symbol NPM1
Gene ID (NCBI) 4869
RRIDAB_2155162
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP06748
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Nucleophosmin (NPM1,B23) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). The transport of proteins across the nuclear envelope is a selective, multistep process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Several cytosolic and nuclear proteins that are central to this process have been identified. The 38 kDa nuclear protein nucleophosmin is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis.