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BCL2 Recombinant antibody, PBS Only

BCL2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3O5

Cat no : 80313-1-PBS

Print datasheet

Synonyms

BCL-2, 3O5

验证数据展示

  • WB analysis using 80313-1-RR (same clone as 80313-1-PBS)

    WB analysis using 80313-1-RR (same clone as 80313-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80313-1-RR (BCL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80313-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 80313-1-RR (same clone as 80313-1-PBS)

    IHC staining of human tonsillitis using 80313-1-RR (same clone as 80313-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80313-1-RR (BCL2 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80313-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BCL2 antibody included
  • IHC staining of human tonsillitis using 80313-1-RR (same clone as 80313-1-PBS)

    IHC staining of human tonsillitis using 80313-1-RR (same clone as 80313-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80313-1-RR (BCL2 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80313-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BCL2 antibody included
  • IF Staining of human tonsillitis using 80313-1-RR (same clone as 80313-1-PBS)

    IF Staining of human tonsillitis using 80313-1-RR (same clone as 80313-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using BCL2 antibody (80313-1-RR, Clone: 3O5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80313-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80313-1-RR

    Affinity and Kinetic Characterization of 80313-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80313-1-RR against Human BCL2 were performed. The affinity constant is 5.06 nM.

Planning an IHC experiment? We recommend our IHCeasy BCL2 Ready-To-Use IHC Kit. BCL2 primary antibody included.

产品信息

80313-1-PBS targets BCL2 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity human
Immunogen BCL2 fusion protein Ag3508 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name B-cell CLL/lymphoma 2
Synonyms BCL-2, 3O5
Calculated Molecular Weight 26 kDa
Observed Molecular Weight26 kDa
GenBank Accession NumberBC027258
Gene Symbol BCL2
Gene ID (NCBI) 596
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP10415
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BCL2 belongs to the Bcl-2 family. It suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. BCL2 regulates cell death by controlling the mitochondrial membrane permeability (PMID: 10365962). It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PMID: 10444588).