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BCL2 Recombinant antibody, PBS Only

BCL2 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3O5

Cat no : 80313-1-PBS

Print datasheet

Synonyms

BCL-2, 3O5

验证数据展示

  • WB analysis using 80313-1-RR (same clone as 80313-1-PBS)

    WB analysis using 80313-1-RR (same clone as 80313-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80313-1-RR (BCL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80313-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 80313-1-RR (same clone as 80313-1-PBS)

    IHC staining of human tonsillitis using 80313-1-RR (same clone as 80313-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80313-1-RR (BCL2 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80313-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BCL2 antibody included
  • IHC staining of human tonsillitis using 80313-1-RR (same clone as 80313-1-PBS)

    IHC staining of human tonsillitis using 80313-1-RR (same clone as 80313-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80313-1-RR (BCL2 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80313-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BCL2 antibody included
  • Affinity and Kinetic Characterization of 80313-1-PBS

    Affinity and Kinetic Characterization of 80313-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 80313-1-RR against Human BCL2 were performed. The affinity constant is 5.06 nM.

Planning an IHC experiment? We recommend our IHCeasy BCL2 Ready-To-Use IHC Kit. BCL2 primary antibody included.

产品信息

80313-1-PBS targets BCL2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen BCL2 fusion protein Ag3508 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name B-cell CLL/lymphoma 2
Synonyms BCL-2, 3O5
Calculated Molecular Weight 26 kDa
Observed Molecular Weight26 kDa
GenBank Accession NumberBC027258
Gene Symbol BCL2
Gene ID (NCBI) 596
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP10415
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BCL2 belongs to the Bcl-2 family. It suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. BCL2 regulates cell death by controlling the mitochondrial membrane permeability (PMID: 10365962). It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PMID: 10444588).