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验证数据展示

  • WB analysis using 80313-1-RR (same clone as 80313-1-PBS)

    WB analysis using 80313-1-RR (same clone as 80313-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80313-1-RR (BCL2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80313-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 80313-1-RR (same clone as 80313-1-PBS)

    IHC staining of human tonsillitis using 80313-1-RR (same clone as 80313-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80313-1-RR (BCL2 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80313-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BCL2 antibody included
  • IHC staining of human tonsillitis using 80313-1-RR (same clone as 80313-1-PBS)

    IHC staining of human tonsillitis using 80313-1-RR (same clone as 80313-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80313-1-RR (BCL2 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80313-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with BCL2 antibody included
  • IF Staining of human tonsillitis using 80313-1-RR (same clone as 80313-1-PBS)

    IF Staining of human tonsillitis using 80313-1-RR (same clone as 80313-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using BCL2 antibody (80313-1-RR, Clone: 3O5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80313-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80313-1-RR

    Affinity and Kinetic Characterization of 80313-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80313-1-RR against Human BCL2 were performed. The affinity constant is 5.06 nM.

BCL2 Recombinant monoclonal antibody, PBS Only

BCL2 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 80313-1-PBS

产品说明书

CloneNo. 3O5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF-P, Indirect ELISA

别名

BCL-2, 3O5

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy BCL2 Ready-To-Use IHC Kit. BCL2 primary antibody included.

产品信息

80313-1-PBS targets BCL2 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag3508

Product name: Recombinant human BCL2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-239 aa of BC027258

Sequence: MAHAGRTGYDNREIVMKYIHYKLSQRGYEWDAGDVGAAPPGAAPAPGIFSSQPGHTPHPAASRDPVARTSPLQTPAAPGAAAGPALSPVPPVVHLTLRQAGDDFSRRYRRDFAEMSSQLHLTPFTARGRFATVVEELFRDGVNWGRIVAFFEFGGVMCVESVNREMSPLVDNIALWMTEYLNRHLHTWIQDNGGWDAFVELYGPSMRPLFDFSWLSLKTLLSLALVGACITLGAYLGHK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 B-cell CLL/lymphoma 2
别名 BCL-2, 3O5
计算分子量 26 kDa
观测分子量26 kDa
GenBank蛋白编号BC027258
基因名称 BCL2
Gene ID (NCBI) 596
RRIDAB_2918886
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP10415
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

BCL2 belongs to the Bcl-2 family. It suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. BCL2 regulates cell death by controlling the mitochondrial membrane permeability (PMID: 10365962). It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PMID: 10444588).

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