BCL2 Recombinant antibody
BCL2 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
3O5
验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, Raji cells, THP-1 cells, HL-60 cells, U-937 cells |
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
80313-1-RR targets BCL2 in WB, IHC, ELISA applications and shows reactivity with Human samples.
Tested Applications | WB, IHC, ELISA Application Description |
Tested Reactivity | Human |
Immunogen | BCL2 fusion protein Ag3508 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | B-cell CLL/lymphoma 2 |
Synonyms | BCL-2, 3O5 |
Calculated Molecular Weight | 26 kDa |
Observed Molecular Weight | 26 kDa |
GenBank Accession Number | BC027258 |
Gene Symbol | BCL2 |
Gene ID (NCBI) | 596 |
RRID | AB_2918886 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P10415 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for BCL2 antibody 80313-1-RR | Download protocol |
IHC protocol for BCL2 antibody 80313-1-RR | Download protocol |
IF protocol for BCL2 antibody 80313-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |