CoraLite® Plus 488-conjugated BCL2 Recombinant antibody

BCL2 Uni-rAbTM Recombinant Antibody for FC (Intra)
Cat No. CL488-80313

产品说明书

CloneNo. 3O5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra)

BCL-2, 3O5

缓冲液配方:  PBS and Azide
PBS and Azide
规格: 

-/ -


经过测试的应用

Positive FC (Intra) detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy BCL2 Ready-To-Use IHC Kit. BCL2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 80313-1-PBS

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-80313 targets BCL2 in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 BCL2 fusion protein Ag3508 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 B-cell CLL/lymphoma 2
别名 BCL-2, 3O5
计算分子量 26 kDa
观测分子量26 kDa
GenBank蛋白编号BC027258
基因名称 BCL2
Gene ID (NCBI) 596
RRIDAB_2934624
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP10415
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BCL2 belongs to the Bcl-2 family. It suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. BCL2 regulates cell death by controlling the mitochondrial membrane permeability (PMID: 10365962). It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PMID: 10444588).

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 BCL2 antibody CL488-80313Download protocol
Standard Protocols
Click here to view our Standard Protocols
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