CoraLite®555-conjugated BCL2 Recombinant antibody
BCL2 Uni-rAbTM Recombinant Antibody for IF-P, FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF-P, FC (Intra)
Conjugate
CoraLite®555 Fluorescent Dye
CloneNo.
3O5
验证数据展示
经过测试的应用
Positive IF-P detected in | human appendicitis tissue |
Positive FC (Intra) detected in | Jurkat cells |
Planning an IHC experiment? We recommend our IHCeasy BCL2 Ready-To-Use IHC Kit. BCL2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 80313-1-PBS
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL555-80313 targets BCL2 in IF-P, FC (Intra) applications and shows reactivity with human samples.
Tested Applications | IF-P, FC (Intra) Application Description |
Tested Reactivity | human |
Immunogen | BCL2 fusion protein Ag3508 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | B-cell CLL/lymphoma 2 |
Synonyms | BCL-2, 3O5 |
Calculated Molecular Weight | 26 kDa |
Observed Molecular Weight | 26 kDa |
GenBank Accession Number | BC027258 |
Gene Symbol | BCL2 |
Gene ID (NCBI) | 596 |
Conjugate | CoraLite®555 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 557 nm / 570 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P10415 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
BCL2 belongs to the Bcl-2 family. It suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. BCL2 regulates cell death by controlling the mitochondrial membrane permeability (PMID: 10365962). It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PMID: 10444588).
实验方案
Product Specific Protocols | |
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IF protocol for CL555 BCL2 antibody CL555-80313 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |