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CoraLite®555-conjugated BCL2 Recombinant antibody

BCL2 Recombinant Antibody for FC (Intra), IF-P

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC (Intra), IF-P

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

3O5

Cat No : CL555-80313

Print datasheet

Synonyms

Apoptosis regulator Bcl 2, B cell CLL/lymphoma 2, BCL2, Oncogene B cell leukemia 2

验证数据展示

  • IF Staining of human appendicitis using CL555-80313

    IF Staining of human appendicitis using CL555-80313

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CoraLite®555 BCL2 antibody (CL555-80313, Clone: 3O5 ) at dilution of 1:200.

  • FC experiment of Jurkat using CL555-80313

    FC experiment of Jurkat using CL555-80313

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human BCL2 (CL555-80313, Clone:3O5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inhuman appendicitis tissue
Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy BCL2 Ready-To-Use IHC Kit. BCL2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 80313-1-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-80313 targets BCL2 in IF, FC (Intra) applications and shows reactivity with Human samples.

Tested Applications FC (Intra), IF-P
Tested Reactivity Human
Immunogen BCL2 fusion protein Ag3508 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name B-cell CLL/lymphoma 2
Synonyms Apoptosis regulator Bcl 2, B cell CLL/lymphoma 2, BCL2, Oncogene B cell leukemia 2
Calculated Molecular Weight 26 kDa
Observed Molecular Weight26 kDa
GenBank Accession NumberBC027258
Gene Symbol BCL2
Gene ID (NCBI) 596
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP10415
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BCL2 belongs to the Bcl-2 family. It suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. BCL2 regulates cell death by controlling the mitochondrial membrane permeability (PMID: 10365962). It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PMID: 10444588).

实验方案

Product Specific Protocols
IF protocol for CL555 BCL2 antibody CL555-80313Download protocol
FC protocol for CL555 BCL2 antibody CL555-80313Download protocol
Standard Protocols
Click here to view our Standard Protocols