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CoraLite® Plus 488-conjugated Bcl-XL Monoclonal antibody

Bcl-XL Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human, canine

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4C12A6

Cat no : CL488-66020

Print datasheet

Synonyms

Apoptosis regulator Bcl X, BCL2L1, Bcl-XL, DKFZp781P2092

验证数据展示

  • IF Staining of HeLa using CL488-66020

    IF Staining of HeLa using CL488-66020

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CL488-66020 (Bcl-XL antibody) at dilution of 1:100.

  • FC experiment of Jurkat using CL488-66020

    FC experiment of Jurkat using CL488-66020

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Bcl-XL (CL488-66020, Clone:4C12A6) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHeLa cells, MDCK cells
Positive FC (Intra) detected inJurkat cells
Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy BCL2L1 Ready-To-Use IHC Kit. BCL2L1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66020-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66020 targets Bcl-XL in IF/ICC, FC (Intra) applications and shows reactivity with human, canine samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, canine
Immunogen Bcl-XL fusion protein Ag18037 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name BCL2-like 1
Synonyms Apoptosis regulator Bcl X, BCL2L1, Bcl-XL, DKFZp781P2092
Calculated Molecular Weight 26 kDa
GenBank Accession NumberBC019307
Gene Symbol Bcl-XL
Gene ID (NCBI) 598
RRIDAB_2883229
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ07817
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

BCL2L1 is a member of the BCL-2 protein family. BCL2L1 is expressed as three isoforms, Bcl-X(L),Bcl-X(s) and Bcl-X(beta), and is located at the outer mitochondrial membrane. The Bcl-XL isoform is a 233 amino acid protein, acting as an apoptotic inhibitor. Bcl-XL can forms heterodimers with BAX, BAK or BCL2, and the heterodimerization with BAX does not seem to be required for anti-apoptotic activity. The Bcl-XS isoform is a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188), acting as an apoptotic activator.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Bcl-XL antibody CL488-66020Download protocol
Standard Protocols
Click here to view our Standard Protocols