CoraLite® Plus 647-conjugated Beta Actin Recombinant antibody

Beta Actin Uni-rAbTM Recombinant Antibody for IF/ICC
Cat No. CL647-81115

产品说明书

CloneNo. 4H1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig, canine

应用

IF/ICC

Actin, ACTB, bate actin, β actin, Actin, cytoplasmic 1

缓冲液配方:  PBS and Azide
PBS and Azide
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected inMDCK cells
Planning an IHC experiment? We recommend our IHCeasy ACTB Ready-To-Use IHC Kit. ACTB primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 81115-1-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-81115 targets Beta Actin in IF/ICC applications and shows reactivity with human, mouse, rat, pig, canine samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat, pig, canine
免疫原 Beta Actin fusion protein Ag14521 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 actin, beta
别名 Actin, ACTB, bate actin, β actin, Actin, cytoplasmic 1
计算分子量 375 aa, 42 kDa
观测分子量 42 kDa
GenBank蛋白编号BC002409
基因名称 Beta Actin
Gene ID (NCBI) 60
RRIDAB_2935094
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP60709
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Beta Actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40, 31, 15 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins. (9173887, 11217076, 10229193 )

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 Beta Actin antibody CL647-81115Download protocol
Standard Protocols
Click here to view our Standard Protocols
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