• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66379-1-Ig (same clone as 66379-1-PBS)

    WB analysis using 66379-1-Ig (same clone as 66379-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66379-1-Ig (Beta Catenin antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 66379-1-Ig (same clone as 66379-1-PBS)

    IHC staining of mouse colon using 66379-1-Ig (same clone as 66379-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Beta Catenin antibody included
  • IHC staining of mouse colon using 66379-1-Ig (same clone as 66379-1-PBS)

    IHC staining of mouse colon using 66379-1-Ig (same clone as 66379-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Beta Catenin antibody included
  • IP experiment of mouse brain using 66379-1-Ig (same clone as 66379-1-PBS)

    IP experiment of mouse brain using 66379-1-Ig (same clone as 66379-1-PBS)

    IP result of anti-Beta Catenin (IP:66379-1-Ig, 4ug; Detection:66379-1-Ig 1:2000) with mouse brain tissue lysate 4400 ug. This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 66379-1-Ig (same clone as 66379-1-PBS)

    IF Staining of MCF-7 using 66379-1-Ig (same clone as 66379-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Beta Catenin antibody (66379-1-Ig, Clone: 1B8A1 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, red). This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 66379-1-Ig (same clone as 66379-1-PBS)

    FC experiment of MCF-7 using 66379-1-Ig (same clone as 66379-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.5 ug Anti-Human Beta Catenin (66379-1-Ig, Clone:1B8A1) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 66379-1-Ig (same clone as 66379-1-PBS)

    IHC staining of human ovary cancer using 66379-1-Ig (same clone as 66379-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:40000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Beta Catenin antibody included
  • IF Staining of human colon cancer using 66379-1-Ig (same clone as 66379-1-PBS)

    IF Staining of human colon cancer using 66379-1-Ig (same clone as 66379-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using Beta Catenin antibody (66379-1-Ig, Clone: 1B8A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66379-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Beta Catenin Monoclonal antibody, PBS Only

Beta Catenin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA
Cat No. 66379-1-PBS

产品说明书

CloneNo. 1B8A1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

别名

Catenin beta-1, Catenin beta 1, beta-Catenin, B-catenin, b cat

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy Beta Catenin Ready-To-Use IHC Kit. Beta Catenin primary antibody included.

产品信息

66379-1-PBS targets Beta Catenin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 catenin (cadherin-associated protein), beta 1, 88kDa
别名 Catenin beta-1, Catenin beta 1, beta-Catenin, B-catenin, b cat
计算分子量 781 aa, 86 kDa
观测分子量 92 kDa
GenBank蛋白编号NM_001904
基因名称 Beta Catenin
Gene ID (NCBI) 1499
ENSEMBL Gene IDENSG00000168036
RRIDAB_2857358
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP35222
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)