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验证数据展示

  • WB analysis using 66379-1-Ig

    WB analysis using 66379-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66379-1-Ig (Beta Catenin antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • IHC staining of mouse colon using 66379-1-Ig

    IHC staining of mouse colon using 66379-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Beta Catenin antibody included
  • IHC staining of mouse colon using 66379-1-Ig

    IHC staining of mouse colon using 66379-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Beta Catenin antibody included
  • IP experiment of mouse brain using 66379-1-Ig

    IP experiment of mouse brain using 66379-1-Ig

    IP result of anti-Beta Catenin (IP:66379-1-Ig, 4ug; Detection:66379-1-Ig 1:2000) with mouse brain tissue lysate 4400 ug.

  • IF Staining of MCF-7 using 66379-1-Ig

    IF Staining of MCF-7 using 66379-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using Beta Catenin antibody (66379-1-Ig, Clone: 1B8A1 ) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, red).

  • FC experiment of MCF-7 using 66379-1-Ig

    FC experiment of MCF-7 using 66379-1-Ig

    1X10^6 MCF-7 cells were intracellularly stained with 0.5 ug Anti-Human Beta Catenin (66379-1-Ig, Clone:1B8A1) (red) labeled with FlexAble CoraLite® Plus 555 Antibody Labeling Kit for Mouse IgG1 (KFA022), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • IHC staining of human colorectal cancer using 66379-1-Ig

    IHC staining of human colorectal cancer using 66379-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:40000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 66379-1-Ig

    IHC staining of human stomach cancer using 66379-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:40000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human appendicitis using 66379-1-Ig

    IHC staining of human appendicitis using 66379-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:40000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 66379-1-Ig

    IHC staining of human pancreas cancer using 66379-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:40000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 66379-1-Ig

    IHC staining of human ovary cancer using 66379-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:40000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 66379-1-Ig

    IHC staining of human breast cancer using 66379-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:40000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 66379-1-Ig

    IHC staining of human liver cancer using 66379-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:40000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 66379-1-Ig

    IHC staining of human placenta using 66379-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:40000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 66379-1-Ig

    IHC staining of human stomach cancer using 66379-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:40000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary cancer using 66379-1-Ig

    IHC staining of human ovary cancer using 66379-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:40000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Beta Catenin antibody included
  • IHC staining of human breast cancer using 66379-1-Ig

    IHC staining of human breast cancer using 66379-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:40000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Beta Catenin antibody included
  • IHC staining of human liver cancer using 66379-1-Ig

    IHC staining of human liver cancer using 66379-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:40000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Beta Catenin antibody included
  • IHC staining of human ovary cancer using 66379-1-Ig

    IHC staining of human ovary cancer using 66379-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:40000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Beta Catenin antibody included
  • IHC staining of human appendicitis using 66379-1-Ig

    IHC staining of human appendicitis using 66379-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:40000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Beta Catenin antibody included
  • IHC staining of human cervical cancer using 66379-1-Ig

    IHC staining of human cervical cancer using 66379-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:40000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Beta Catenin antibody included
  • IHC staining of human pancreas cancer using 66379-1-Ig

    IHC staining of human pancreas cancer using 66379-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:40000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Beta Catenin antibody included
  • IHC staining of human colorectal cancer using 66379-1-Ig

    IHC staining of human colorectal cancer using 66379-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:40000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Beta Catenin antibody included
  • IHC staining of human stomach cancer using 66379-1-Ig

    IHC staining of human stomach cancer using 66379-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:40000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Beta Catenin antibody included
  • IHC staining of human stomach cancer using 66379-1-Ig

    IHC staining of human stomach cancer using 66379-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:40000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Beta Catenin antibody included
  • IHC staining of human breast cancer using 66379-1-Ig

    IHC staining of human breast cancer using 66379-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Beta Catenin antibody included
  • IF Staining of human colon cancer using 66379-1-Ig

    IF Staining of human colon cancer using 66379-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using Beta Catenin antibody (66379-1-Ig, Clone: 1B8A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse liver using 66379-1-Ig

    IF Staining of mouse liver using 66379-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Beta Catenin antibody (66379-1-Ig, Clone: 1B8A1 ) at dilution of 1:400 and CoraLite®647-conjugated F(ab')2 Fragment Goat Anti-Mouse IgG (H+L) (SA00014-10), Glutamine Synthetase antibody (11037-2-AP, green). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat colon using 66379-1-Ig

    IHC staining of rat colon using 66379-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Beta Catenin antibody included
  • IHC staining of mouse colon using 66379-1-Ig

    IHC staining of mouse colon using 66379-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 66379-1-Ig (Beta Catenin antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Beta Catenin antibody included
  • Featured Product
  • KD/KO Validated

Beta Catenin Monoclonal antibody

Beta Catenin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA
Cat No. 66379-1-Ig

产品说明书

CloneNo. 1B8A1
引用文献(170)

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig and More (3)

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA and More (2)

别名

1B8A1, b cat, B-catenin, beta-Catenin, Catenin beta 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, pig brain tissue, rat brain tissue, mouse brain tissue, HeLa cells, HEK-293 cells, MCF-7 cells, LNCaP cells, HSC-T6 cells, NIH/3T3 cells, rabbit brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse colon tissue, human breast cancer tissue, human colon tissue, human ovary cancer tissue, human skin cancer tissue, rat colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman colon cancer tissue, human skin cancer tissue, mouse liver tissue
Positive IF/ICC detected inMCF-7 cells, human liver cancer tissue, human skin cancer tissue
Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy Beta Catenin Ready-To-Use IHC Kit. Beta Catenin primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:10000-1:40000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:750-1:3000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66379-1-Ig targets Beta Catenin in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human, mouse, rat, pig
文献引用反应性human, mouse, rat, pig, rabbit, chicken, sheep
免疫原

Peptide

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 catenin (cadherin-associated protein), beta 1, 88kDa
别名 1B8A1, b cat, B-catenin, beta-Catenin, Catenin beta 1
计算分子量 781 aa, 86 kDa
观测分子量 92 kDa
GenBank蛋白编号NM_001904
基因名称 Beta Catenin
Gene ID (NCBI) 1499
ENSEMBL Gene IDENSG00000168036
RRIDAB_2857358
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP35222
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222)

实验方案

Product Specific Protocols
FC protocol for Beta Catenin antibody 66379-1-IgDownload protocol
IF protocol for Beta Catenin antibody 66379-1-IgDownload protocol
IHC protocol for Beta Catenin antibody 66379-1-IgDownload protocol
IP protocol for Beta Catenin antibody 66379-1-IgDownload protocol
WB protocol for Beta Catenin antibody 66379-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Adv Mater

Noninvasive Optogenetics Realized by iPSC-Derived Tentacled Carrier in Alzheimer's Disease Treatment

Authors - Yuewen Zhai
View Article

Nat Commun

Cholecystectomy-related gut microbiota dysbiosis exacerbates colorectal tumorigenesis

Authors - Bo Tang
View Article
rat,mouseWB

Acta Pharm Sin B

Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2.

Authors - Xunxun Wu
View Article
humanWB

J Exp Clin Cancer Res

Membrane RRM2-positive cells represent a malignant population with cancer stem cell features in intrahepatic cholangiocarcinoma

Authors - Yongzhi Zhao
  • KD Validated
View Article
humanIF

Research (Wash D C)

The Mechanics of Tumor Cells Dictate Malignancy via Cytoskeleton-Mediated APC/Wnt/β-Catenin Signaling

Authors - Xi Chen
View Article
humanIF

Acta Biomater

Microenvironmental reprogramming of human dermal papilla cells for hair follicle tissue engineering

Authors - Zhen Liu
View Article
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